Genotyping and primer details


Genotyping and primer details for SALK_085672c - At1g17430
Line ID SALK_085672c
Locus At1g17430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5983022 of line SALK_085672c Chr1:5983022
   
Insertion allele amplicon LBb3 - 50vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 50vi: 5'-AAGTGAAAGCAAACAAACGAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5983457
   
wt amplicon (over InsPos) 62vw - 50vi
wt amplicon start Chr1:5982334
wt amplicon end Chr1:5983457
wt amplicon size 1124 bp
Locus specific primer 50vi: 5'-AAGTGAAAGCAAACAAACGAGAAT-3'
2nd locus specific primer 62vw: 5'-CTTTTCCGTCGTTATCTCTCAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11