Genotyping and primer details


Genotyping and primer details for SALK_022827c - At1g32690
Line ID SALK_022827c
Locus At1g32690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11821651 of line SALK_022827c Chr1:11821651
   
Insertion allele amplicon LBb3 - 29um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29um: 5'-TATACAATGGAGACCGTTGTTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11821087
   
wt amplicon (over InsPos) 17vb - 29um
wt amplicon start Chr1:11821087
wt amplicon end Chr1:11821937
wt amplicon size 851 bp
Locus specific primer 29um: 5'-TATACAATGGAGACCGTTGTTGAA-3'
2nd locus specific primer 17vb: 5'-AAAGAGAAGTCGTTTCCAAAGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11