Genotyping and primer details


Genotyping and primer details for SALK_015099c - At1g10650
Line ID SALK_015099c
Locus At1g10650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3524273 of line SALK_015099c Chr1:3524273
   
Insertion allele amplicon LBb3 - 72by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 72by: 5'-GAAGCCGAAAGCATTAGTAACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3525033
   
wt amplicon (over InsPos) 87dy - 72by
wt amplicon start Chr1:3524090
wt amplicon end Chr1:3525033
wt amplicon size 944 bp
Locus specific primer 72by: 5'-GAAGCCGAAAGCATTAGTAACAAC-3'
2nd locus specific primer 87dy: 5'-CTCTTTTTCCTTCTGTTCCATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11