Genotyping and primer details


Genotyping and primer details for 104B08 - At1g07705
Line ID 104B08
Locus At1g07705 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2382958 of line 104B08 Chr1:2382958
   
Insertion allele amplicon 8474 - IW45
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IW45: 5'-TTTGGCTCTGCTTAAAAGTTTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2382639
   
Insertion allele amplicon 3144 - IW46
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer IW46: 5'-TCGACTGATTAGGAAATTCAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2383246
   
wt amplicon (over InsPos) IW45 - IW46
wt amplicon start Chr1:2382639
wt amplicon end Chr1:2383246
wt amplicon size 607 bp
Locus specific primer IW46: 5'-TCGACTGATTAGGAAATTCAAACA-3'
2nd locus specific primer IW45: 5'-TTTGGCTCTGCTTAAAAGTTTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 02.09.2016 15:25