Genotyping and primer details


Genotyping and primer details for SALK_072621c - At3g52450
Line ID SALK_072621c
Locus At3g52450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19441051 of line SALK_072621c Chr3:19441051
   
Insertion allele amplicon LBb3 - 20gg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20gg: 5'-TCGAGCTTTTAATGGACGATACTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19441343
   
wt amplicon (over InsPos) 19hs - 20gg
wt amplicon start Chr3:19440695
wt amplicon end Chr3:19441343
wt amplicon size 649 bp
Locus specific primer 20gg: 5'-TCGAGCTTTTAATGGACGATACTT-3'
2nd locus specific primer 19hs: 5'-AATAGCCATGTAACCTGTGAATCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11