Line ID |
SALK_147407 |
Locus |
At1g52550 [Araport] [TAIR] [MIPS] [SIGnAL] |
Deduced position of insertion |
Chr1:19573162 |
| |
Insertion allele amplicon |
LBb3 - 01cb |
T-DNA primer |
LBb3: 5'-ATTTTGCCGATTTCGGAAC-3' |
Locus specific primer |
01cb: 5'-AGCACACACCAAACACATCTAGTC-3' |
Sequences from confirmation amplicon |
show confirmation sequences |
Genomic end of ins. amplicon |
Chr1:19572887 |
| |
Insertion allele amplicon |
LBb3 - 06cq |
T-DNA primer |
LBb3: 5'-ATTTTGCCGATTTCGGAAC-3' |
Locus specific primer |
06cq: 5'-TGAAGGTGGTTTAACTGCTATTGA-3' |
Sequences from confirmation amplicon |
show confirmation sequences |
Genomic end of ins. amplicon |
Chr1:19573623 |
| |
Insertion allele amplicon |
LBb3 - 16wp |
T-DNA primer |
LBb3: 5'-ATTTTGCCGATTTCGGAAC-3' |
Locus specific primer |
16wp: 5'-ACCTCACCTTGGTGAATCTGTACT-3' |
Sequences from confirmation amplicon |
show confirmation sequences |
Genomic end of ins. amplicon |
Chr1:19572339 |
| |
Insertion allele amplicon |
LBb3 - SC04 |
T-DNA primer |
LBb3: 5'-ATTTTGCCGATTTCGGAAC-3' |
Locus specific primer |
SC04: 5'-TCAAGATCGATTACTGTTCATTGC-3' |
Sequences from confirmation amplicon |
show confirmation sequences |
Genomic end of ins. amplicon |
Chr1:19573570 |
| |
wt amplicon (over InsPos) |
01cb - 06cq |
wt amplicon start |
Chr1:19572887 |
wt amplicon end |
Chr1:19573623 |
wt amplicon size |
737 bp |
Locus specific primer |
06cq: 5'-TGAAGGTGGTTTAACTGCTATTGA-3' |
2nd locus specific primer |
01cb: 5'-AGCACACACCAAACACATCTAGTC-3' |
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Note |
The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details. |