Genotyping and primer details


Genotyping and primer details for SALK_151939 - At1g53850
Line ID SALK_151939
Locus At1g53850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20104861 of line SALK_151939 Chr1:20104861
   
Insertion allele amplicon LBb3 - 67cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 67cs: 5'-AAAAGGGATTGTTGAATTTTGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20105437
   
wt amplicon (over InsPos) 30dw - 67cs
wt amplicon start Chr1:20104426
wt amplicon end Chr1:20105437
wt amplicon size 1012 bp
Locus specific primer 67cs: 5'-AAAAGGGATTGTTGAATTTTGTTG-3'
2nd locus specific primer 30dw: 5'-GAAAAACAATTGGGAAAAAGTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11