Sequences from confirmation amplicon


Confirmation sequences for line 435H12 and hit At1g02690 are shown.
Line ID 435H12
Locus At1g02690 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:587011
Primers used for PCR JG49 - 8474, show primer details
   
Sequence Name 26-K034066-022-435-H12C-8409
GenBank Accession LN497823 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K034066-022-435-H12C-8409
TGAAGCCATTTCCCGGAGCATTACCACTTNTGGTTTCCGAATTGGAAACCGGAAGACTGA
ACATTCTCAGGAGCATCCACACCGCCAATATCTTCCTCCTCGTCATCTGCAGCCCAATAC
GACTCCAGGATTTTGACTGCTTTCTCATAAATCTCATTATTATCGTGACTCTGAAGATTC
TCAATCTTGTCCAAACCATCTGCGTCTTCAATCATCTGCGCGTACAGATTATCGTTTCCT
GTGTTGCCTAGATTCTTCTCGGCTTCACCTACTTTGAGAATGTTTTCTAGACCTTCCAAA
GTCACTGTGACAACCCTTGGATCGGGACACGGTAGGAGATCACACAGGGGTCTGATGCAG
CCTTGGCTCACGAGAAACCTGCAAGGATATAAGAGGTTCTATAACAAGAATCACTGAAAA
AAAAACTAAAAAGAGACAGAAACAATGAGATTTGTTGTACTTGATTTGGTCATGATTGCC
ACCAGAAGTTGCATTAGAGATTGCCCAAACAGCTTCTTTCTTAATTTCGAATTCACCGAT
CTCAAGCAGGTTAATCAGAGGCCGAATAATGCCTGCCTGAAACACTTCCTGCACAACAAA
TTTATCGATGTTAAATCCAAATGTATATACGACTTCTGGTTTTAAATTATAAGAAAAGAT
ATTGGTTTTTGAACATTGTTATTTGTTAATAATGTATATAACTCACTTGAATTTGACTTG
TGTTCC
   
Sequence Name 42-K034066-022-435-H12C-JG49
GenBank Accession LN504372 [GenBank]
Sequencing primer gene specific primer, JG49: 5'-CTAACATTACAGCTGGGAACACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K034066-022-435-H12C-JG49
ATATANCATTATTAACAAATAACAATGGTTCAAAAACCAATANTCTTTTCTTATAATTTA
AAACCAGAAGTCGTATATACATTTGGATTTAACATCGATAAATTTGTTGTGCAGGAAGTG
TTTCAGGCAGGCATTATTCGGCCTCTGATTAACCTGCTTGAGATCGGTGAATTCGAAATT
AAGAAAGAAGCTGTTTGGGCAATCTCTAATGCAACTTCTGGTGGCAATCATGACCAAATC
AAGTACAACAAATCTCATTGTTTCTGTCTCTTTTTAGTTTTTTTTTCAGTGATTCTTGTT
ATAGAACCTCTTATATCCTTGCAGGTTTCTCGTGAGCCAAGGCTGCATCAGACCCCTGTG
TGATCTCCTACCGTGTCCCGATCCAAGGGTTGTCACAGTGACTTTGGAAGGTCTAGAAAA
CATTCTCAAAGTAGGTGAAGCCGAGAAGAATCTAGGCAACACAGGAAACGATAATCTGTA
CGCGCAGATGATTGAAGACGCAAATGGTTTGGACAAGATTGAGAATCTTCAGAGTCACGA
TAATAATGAGATTTATGAGAAAGCAGTCAAAATCCTGGAGTCGTATTGGGCTGCAGATGA
CGAGGAGGAAGATATTGGCGGTGTGGATGCTCCTGAGAATGTTCAGTCTTCCGGTTTCCA
ATTCGGAAACCAAAGTGGTAATGCTCCGGGAAATGGCTTCAATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11