Sequences from confirmation amplicon


Confirmation sequences for line 187B05 and hit At1g05630 are shown.
Line ID 187B05
Locus At1g05630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1683693
Primers used for PCR PB11 - 8474, show primer details
   
Sequence Name 47-K102431-0022-187-B05C-8409
GenBank Accession LN506161 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K102431-0022-187-B05C-8409
ATTTACAATTGAATATATATTAATTACCAAGTGAGAAAAATAAAAGCCTACTTACCAGAT
TGAGAATGAGAGTGACTGCACAGCCCACACTTTAGCTCTTACTGAATCAGCTAACAAAAA
CTTGACTTCTGAGGAAGATATGCTACATGTCCCATTTACAGTAACTTGGCTCCTTAGGTC
AATGGCAGATCTCTCCACTAATAGAGCAGCCATGTGTTTCTCCTCCGGTTTAAGCAGCAG
AGATTTCTCTAACGTATCCCAAGGCCATATTTTTATAACACCTCCTTCAGAACATGACCA
CATATCACCTACATCACAACCACAAGCATAAGCAAGATT
   
Sequence Name 47-K102432-0022-187-B05C-PB11
GenBank Accession LN506162 [GenBank]
Sequencing primer gene specific primer, PB11: 5'-TAATCTTGCTTATGCTTGTGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K102432-0022-187-B05C-PB11
AGTGTTATAAAAATATGGCCTTGGGATACGTTAGAGAAATCTCTGCTGCTTAAACCGGAG
GAGAAACACATGGCTGCTCTATTAGTGGAGAGATCTGCCATTGACCTAAGGAGCCAAGTT
ACTGTAAATGGGACATGTAGCATATCTTCCTCAGAAGTCAAGTTTTTGTTAGCTGATTCA
GTAAGAGCTAAAGTGTGGGCTGTGCAGTCACTCTCATTCTCAATCTGGTAAGTAGGCTTT
TATTTTTCTCACTTGGTAATTAATATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR PB12 - 8474, show primer details
   
Sequence Name 87-K112971-0022-187-B05C-PB12
GenBank Accession LN514783 [GenBank]
Sequencing primer gene specific primer, PB12: 5'-ATTTCCATCCCACTGAACAATTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K112971-0022-187-B05C-PB12
TGTTCATCTACGCTACACGATAGCTTCTGTTTTCCTAGTATCTTCCGAAAACGCTCCTGC
ACTTCTAGTGGCGACTCGGCGAACAGCTCCTGCCGCTCCCATTATGGCATTACGTGATCG
CTGCAGAAAGCCCTGTGGCTTTTCCCTTTTTGAAGCTGAGAAGAACTTCAGTTTCATTTC
ATCATCAACTTGTTGGTCTTGTATAGGTGGCAGATCCCCACGATTCTCAACTTGCCCGTC
AACATTTAAAACTTTCAGAAGGTCTTTACTCCGAGCATCCCTGCGCACATAAATCTCTCA
TAATGATCATCAACAAGTGACTGAAAAATACATGTAAATGTATGAAGCCATTCTCATCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCGCACGGTTATT
   
Sequence Name 85-K112970-0022-187-B05C-8409
GenBank Accession LN514567 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K112970-0022-187-B05C-8409
ATTTCATTGAATTATCCTGATGAGAATGGCTTCATACATTTACATGTATTCTTCTGTCAC
TTGTTGATGATCATTATGAGAGATTTATGTGCGCAGGGATGCTCGGAGTAAAGACCTTCT
GAAAGTTTTAAATGTTGACGGGCAAGTTGAGAATCGTGGGGATCTGCCACCTATACAAGA
CCAACAAGTTGATGATGAAATGAAACTGAAGTTCTTCTCAGCTTCAAAAAGGGAAAAGCC
ACAGGGCTTTCTGCAGCGATCACGTAATGCCATAATGGGAGCGGCAGGAGCTGTTCGCCG
AGTCGCCACTAGAAGTGCAGGAGCGTTTTCGGAAGATACTAGGAAAACAGAAGCTATCGT
GTTAGCTGTAGATGGAACAATTTGGACTGGAAGCATAAGTGGCCTAATTGTTCAGTGGNA
TGGAAA

Last Updated on 06.12.2018 16:11