Sequences from confirmation amplicon


Confirmation sequences for line 283B02 and hit At1g10417 are shown.
Line ID 283B02
Locus At1g10417 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3427550
Primers used for PCR CQ28 - 8474, show primer details
   
Sequence Name 44-K105920-0022-283-B02C-CQ28
GenBank Accession LN505188 [GenBank]
Sequencing primer gene specific primer, CQ28: 5'-AACACACCAGCATTTCACTAACTG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K105920-0022-283-B02C-CQ28
ATTCTCTAGCTATTTTATCCCTTAGATATAGTATAAGATTTTGACAGGATTGAGATTTTA
TGAGAAAAGCACCTGGTAAGCATTTAAGAAACTTTCTCGCGATTGAAGCAAATTTTGGAT
AGTTGTCTTCAATTCACTCTGCTTGTTTTCCAGCAAACTATAGTCGTTCTGAAGATAACT
CACCTGCAAAATCAATTAGAAACTTTCACTAACTCCATGTGAAATAGATTCGCAGAAGAG
AAGAATCTTCCAAACCTTTCTCTCGAGTGCCTCCTGTGTTTCTCTAGAAGAAGCTAAATC
AGCTTGAAGCTCCTGCACTTTCCTGATGCAGTCCTCCTCCGTTTGCTTCTGTTTCTGTAG
CGAAAGCACCAAACAAAATGATCACCATCTACGAATCAGAAACGAAACAAAAACGAGAGC
TATGAACAGTAACATTGATCGAAGAACCTGATTCGCAAGAGTAATGTGATCTGTGACTGA
TCGCTCCCTTTCCTGCAAAAACGCCGGTNGTAAAGTGGAAGAAGACGAAGAAGAAGAAGA
GAGAAACTGGTGGAGCGAAGAAGATACACAATCCATAGATGTATAGAAACCTCTTTCTCC
TTACACGCAGATCTCGCACTTCTCCGATCATTGACTGAAGCTGCAATTTGAATTTTGACA
ACTTGAGCATCTCCNTTTTTGGTTGGTTTCTTCCTCCGATTTTGAATTTCGACCGCCAGA
AAATCTCTTCTTTGTGGAAAAAAAATCAATGTCAACGAGAGGAAAATTAGGTCGTCGGAA
TTGTCAAACAATGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGT
   
Sequence Name 44-K105921-0022-283-B02C-8409
GenBank Accession LN505189 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K105921-0022-283-B02C-8409
TAATTTTCCTCTCGTTGACTTGATTTTTTTTCCACAAGAAGAGATTTTCTGGCGGTCGAA
ATTCAAAATCGGAGGAAGAAACCAACCAAAAATGGAGATGCTCAAGTTGTCAAAATTCAA
ATTGCAGCTTCAGTCAATGATCGGAGAAGTGCGAGATCTGCGTGTAAGGAGAAAGAGGTT
TCTATACATCTATGGATTGTGTATCTTCTTCGCTCCACCAGTTTCTCTCTTCTTCTTCTT
CGTCTTCTTCCACTTTACTACCGGCGTTTTTGCAGGAAAGGGAGCGATCAGTCACAGATC
ACATTACTCTTGCGAATCAGGTTCTTCGATCAATGTTACTGTTCATAGCTCTCGTTTTTG
TTTCGTTTCTGATTCGTAGATGGTGATCATTTTGTTTGGTGCTTTCGCTACAGAAACAGA
AGCAAACGGAGGAGGACTGCATCAGGAAAGTGCAGGAGCTTCAAGCTGATTTAGCTTCTT
CTAGAGAAACACAGGAGGCACTCGAGAGAAAGGTTTGGAAGATTCTTCTCTTCTGCGAAT
CTATTTCACATGGAGTTAGTGAAAGTTTCTAATTGATTTTGCAGGTGAGTTATCTTCAGA
ACGACTATNGTTTGCTGGAAAACAAGCAGAGTGAATTGAAGACAACTATCCAAAATTTGC
TTCAATCGCGAGAAAGTTTCTTAAATGCTTACCAGGTGCTTTTCTCATAAAATCTCAATC
CTGTCAAAATCTTATACTATATCTAAGGGATAAAAATAGCTNAGAGAATCATCTCCATGA
TATGGAACTGTGTAACTTCAGTTAGTG

Last Updated on 06.12.2018 16:11