Sequences from confirmation amplicon


Confirmation sequences for line 908D02 and hit At1g20515 are shown.
Line ID 908D02
Locus At1g20515 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7106187
Primers used for PCR 01jn - 8474, show primer details
   
Sequence Name 61-K113193-0022-908-D02C-01jn
GenBank Accession LN510569 [GenBank]
Sequencing primer gene specific primer, 01jn: 5'-GAATCTCCTCACAGTTTCTCATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113193-0022-908-D02C-01jn
AAAAGACACACCAGTGGAAATACAGACATTACCTAGTGATAATCTCTAAGAGAAGGGCAA
TAACACAATTTAGCCAAGAAGGAGGAAGAAACAGAAACCAAACCTTCAAATTACGAAACA
GAGGAACAAAAAAAAACGAACAGAGTCTTACCACCATCTCTGTGCAATGTGCATGATCAA
GAACCTAAACTCATTAGTAAACTAAATATAAGCATTCACAAGGAGTTCATGGGAAGATAA
AAACTGTGTGATAGCTTTGGCAATTATTCAATTACAACTACAACTCAAATTTTTAAACCA
AGTCGCATTACTTCTTTGATCAAAATGTATGAGAAACAAAGATAAATTGCCTCTGATCCT
GATCAGTTCCTCTTATTATCAAATTGATTCATAAATCCAAGGTAAGTAGTAATCACCCGA
ATTCTAGAAACATATCCCTAACATGCTCAATTACNGAGAGACAAAGAGAGAGAGGTTCAC
CGGAACCCTACCTTCCGGCTTCACATTTCAGTCAGGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 24-K113192-0022-908-D02C-8409
GenBank Accession LN497068 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K113192-0022-908-D02C-8409
TTTCATTGAATATATCCTGACTGAAATGTGAAGCCGGAAGGTAGGGTTCCGGTGAACCTC
TCTCTCTTTGTCTCTCTGTAATTGAGCATGTTAGGGATATGTTTCTAGAATTCGGGTGAT
TACTACTTACCTTGGATTTATGAATCAATTTGATAATAAGAGGAACTGATCAGGATCAGA
GGCAATTTATCTTTGTTTCTCATACATTTTGATCAAAGAAGTAATGCGACTTGGTTTAAA
AATTTGAGTTGTAGTTGTAATTGAATAATTGCCAAAGCTATCACACAGTTTTTATCTTCC
CATGAACTCCTTGTGAATGCTTATATTTAGTTTACTAATGAGTTTAGGTTCTTGATCATG
CACATTGCACAGAGATGGTGGTAAGACTCTGTTCGTTTTTTTTTGTTCCTCTGTTTCGTA
ATTTGAAGGTTTGGTTTCTGTTTCTTCCTCCTTCTTGGCTAAATTGTGTTATTGCCCTTC
TCTTAGAGATTATCACTAGGTAATGTCTGTATTTCCACTGGTGTGTCTTTTTGTTTTATA
ACATTCACTTTTTGAGCAATAATAAACAATGGATGAGAAACTGTG

Last Updated on 06.12.2018 16:11