Sequences from confirmation amplicon


Confirmation sequences for line 011D02 and hit At1g23010 are shown.
Line ID 011D02
Locus At1g23010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8148991
Primers used for PCR 45kb - 3144, show primer details
   
Sequence Name 09-K113214-0022-011-D02C-3144
GenBank Accession LN490258 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113214-0022-011-D02C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCCGGGGATCAGATTGTCGGGTAAACCTAAGACATTATAGATTTGACCAACACT
ATAGATTAGATTATAGTGTCCATTTACAATTGATTCATAGGGCAAACCATTGACTAACAA
ATGAGTTGGCTCATCAGAGTTACTCACATACTCGTACATAGAGATGTAACGCGTGAGAAC
GGCGTTTGACACGTCAGCATTCGGATAATTGATGAGCTTCTTGGGAATTGTACATGTGTC
ATCCTCTGATTCATTGTTGATGATAAATTTCATTACCTTGCCATTTTCTTCGTTGACAGG
ATCACCACTAGGGTAAGGATATGGTGCGTCATTAGCAAGCACCACCGTTCTCGACGGTGA
TTTGTAGAAATCAACAACAACGTCTACAATTTCTGATGGAGATAAGAGAATCGATTTGGT
CATCACCGGCTTTGACAAATACGCTGAATCAAACCCCACAACGATGAAATCTAGACCGTT
GGAGAAGAAAAACTTGAAAAACCTAGCGTTGCTTGCGTTTATGATACGGAATCTGTATTT
CCTACGTCGGACGTT
   
Sequence Name 09-K113215-0022-011-D02C-45kb
GenBank Accession LN490259 [GenBank]
Sequencing primer gene specific primer, 45kb: 5'-ATAATCGTTAATGGAAAAGCATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K113215-0022-011-D02C-45kb
ATTCCGTATCATAAACGCAAGCAACGCTAGGTTTTTCAGCTTTTTCTTCTCCATCGGTCT
AGATTTCATCGTTGTGGGGTCTGATTCAGCGTATTTGTCAAAGCCGGTGATGACCAAATC
GATTCTCTTATCTCCATCAGAAATTGTAGACGTTGTTGTTGATTTCTACAAATCACCGTC
GAGAACGGTGGTGCTTGCTAATGACGCACCATATCCTTACCCTAGTGGTGATCCTGTCAA
CGAAGAAAATGGCAAGGTAATGAAATTTATCATCAACAATGAATCAGAGGATGACACATG
TACAATTCCCAAGAAGCTCATCAATTATCCGAATGCTGACGTGTCAAACGCCGTTCTCAC
GCGTTACATCTCTATGTACGAGTATGTGAGTAACTCTGATGAGCCAACTCTTTTGTTAGT
CAATGGTTTGCCCTATGAATCAATTGTAAATGGACACTATAATCTAATCTATAGTGTTGG
TCAAATCTATAATGTCTTAGGTTTACCCGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA

Last Updated on 06.12.2018 16:11