Sequences from confirmation amplicon


Confirmation sequences for line 044E09 and hit At1g27480 are shown.
Line ID 044E09
Locus At1g27480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9544404
Primers used for PCR 02ok - 8474, show primer details
   
Sequence Name 33-K114805-0022-044-E09C-02ok
GenBank Accession LN501106 [GenBank]
Sequencing primer gene specific primer, 02ok: 5'-AAACCATCCACCACTCTTCTTATG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K114805-0022-044-E09C-02ok
CAGAAACACTTGGCTCGTATTCTCTGTTCAGCCGTACCTCTAGCTGGTTACCTCCGTTTC
CTGGAACCAGAATCAAAGGGTACACGTTGCTACCCACAGCTTGACACATCGAGGTCATCG
TCACCACAACGAGTATCGCTATGACTACCGAATAATGTGAAGATATTTTTTTCATTTTCG
TTCTAAGAAACAGACTCTCATGGTCATGGATCTATGCAGAAAGCTGGAGATTTGAAGAAA
AAGGTCCATTGAATTTGAAAAACAGAGTAGTATCTTAAAACGTAAGGCTTAAGATAAGTA
GTATATGGTGGATATGGAACCCGCGTAATCATCTAGAGGCTCTACAAATATTTATTTTGT
ATTTCCTTCTTATTTTGTATTTGCCTACGTGGCATTATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 09-K114804-0022-044-E09C-8409
GenBank Accession LN490298 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K114804-0022-044-E09C-8409
GCCACGTCAGGCAATACAAAATAAGAAGGAAATACAAAATAAATATTTGTAGAGCCTCTA
GATGATTACGCGGGTTCCATATCCACCATATACTACTTATCTTAAGCCTTACGTTTTAAG
ATACTACTCTGTTTTTCAAATTCAATGGACCTTTTTCTTCAAATCTCCAGCTTTCTGCAT
AGATCCATGACCATGAGAGTCTGTTTCTTAGAACGAAAATGAAAAAAATATCTTCACATT
ATTCGGTAGTCATAGCGATACTCGTTGTGGTGACGATGACCTCGATGTGTCAAGCTGTGG
GTAGCAACGTGTACCCTTTGATTCTGGTTCCAGGAAACGGAGGTAACCAGCTAGAGGTAC
GGCTGGACAGAGAATACAAGCCAAGTAGTGTCTGGTGTAGCAGCTGGTTATATCCAATTC
ATAAGAA

Last Updated on 06.12.2018 16:11