Sequences from confirmation amplicon


Confirmation sequences for line 723H05 and hit At1g51990 are shown.
Line ID 723H05
Locus At1g51990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19331360
Primers used for PCR CR47 - 8474, show primer details
   
Sequence Name 31-K107199-0022-723-H05C-CR47
GenBank Accession LN500188 [GenBank]
Sequencing primer gene specific primer, CR47: 5'-CCACATTTTGCAATCACATTATTC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K107199-0022-723-H05C-CR47
GATTTCTCTCAACTTCCGGAGGATCAGCGAAGAAGAAGATATGTTATTAGCCATACAACT
AGGCGGGCTAAACTTTGTCCCGTACATTGTAAAAACTGCTAGAGAACTAGATTTGTTCGA
GATTATGGCTAAGGCCAGACCTTTAGGGTCCTATCTCTCACCGGTGGATTTGGCTTCCAT
GGCTGCGCCGAAAAATCCACACGCTCCAATGATGATTGATAGATTGCTACGTTTCCTTGT
GGCATATTCGGTGTGCACGTGTAAGTTGGTGAAAGATGAAGAAGGACGAGAGTCTAGAGC
ATACGGATTAGGAAAAGTTGGAAAGAAGTTGATTAAGGATGAAGACGGATTTTCAATTGC
GCCCTATGTGCTAGCTGGCTGTACAAAAGCCAAGGGAGGTGTATGGTATAATAGGCTAAT
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCACGGTTATTA
   
Sequence Name 15-K107198-0022-723-H05C-8409
GenBank Accession LN492998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K107198-0022-723-H05C-8409
CGGAATGAGCCATTTACAATTGAATATATTAGCCTATTNACCATACACCTCCCTTGGCTT
TTGTACAGCCAGCTAGCACATAGGGCGCAATTGAAAATCCGTCTTCATCCTTAATCAACT
TCTTTCCAACTTTTCCTAATCCGTATGCTCTAGACTCTCGTCCTTCTTCATCTTTCACCA
ACTTACACGTGCACACCGAATATGCCACAAGGAAACGTAGCAATCTATCAATCATCATTG
GAGCGTGTGGATTTTTCGGCGCAGCCATGGAAGCCAAATCCACCGGTGAGAGATAGGACC
CTAAAGGTCTGGCCTTAGCCATAATCTCGAACAAATCTAGTTCTCTAGCAGTTTTTACAA
TGTACGGGACAAAGTTTAGCCCGCCTAGTTGTATGGCTAATAACATATCTTCTTCTTCGC
TTGATCCTCCGGAAGTTTGTAGAGAAATCATATGTAACTATTGTTTCGGTTGAATAATGT
GATTGCAAAATGTGG

Last Updated on 06.12.2018 16:11