Sequences from confirmation amplicon


Confirmation sequences for line 255G08 and hit At1g52920 are shown.
Line ID 255G08
Locus At1g52920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19710909
Primers used for PCR 26sm - 8474, show primer details
   
Sequence Name 19-K116405-0022-255-G08C-26sm
GenBank Accession LN494916 [GenBank]
Sequencing primer gene specific primer, 26sm: 5'-TTTAGCTTTCTCAGCTTCACCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116405-0022-255-G08C-26sm
CTTTGGGTCTATCCGGAGCAGCTCTCGCTCTTTTTCACCGGCGCCGGAGCTTCCGTCTCA
CTGTGCTTCCGCTTCCTCCCCATTTTTTTGTTTTTCCGCGTAGAGACGTTTTCTCTAGGG
TTTTAGCAAGTGTTTTAAGGTCAGAGACGAAACAGAGAGAAAAAATCATGGGCCGGTCAT
TATTGGGCTTAACTTAAGGCCCATTAATAATGGTAACGATGTGTCACTTCGTCACTTCGT
CGTTAGTCTCTGTTCTTCGCGGTCCTCTGCAGCAACCGCTGAGAGAAAGAGAGATATGGG
AGAACGGTTTTTCCGAAATGAGATGCCGGAGTTTGTACCGGAAGATTTATCCGGAGAAGA
AGAAACTGTTACCGAATGTAAAGATTCGTTGACGAAACTGCTATCACTTCCGTACAAATC
ATTCTCAGAGAAGCTTCACAGATCCGGGAAATATACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCCGCAGCGTTATTAT
   
Sequence Name 09-K116404-0022-255-G08C-8409
GenBank Accession LN490350 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116404-0022-255-G08C-8409
AGCTTCTCTGAGATGATTTGTACGGAAGTGATAGCAGTTTCGTCAACGAATCTTTACATT
CGGTAACAGTTTCTTCTTCTCCGGATAAATCTTCCGGTACAAACTCCGGCATCTCATTTC
GGAAAAACCGTTCTCCCATATCTCTCTTTCTCTCAGCGGTTGCTGCAGAGGACCGCGAAG
AACAGAGACTAACGACGAAGTGACGAAGTGACACATCGTTACCATTATTAATGGGCCTTA
AGTTAAGCCCAATAATGACCGGCCCATGATTTTTTCTCTCTGTTTCGTCTCTGACCTTAA
AACACTTGCTAAAACCCTAGAGAAAACGTCTCTACGCGGAAAAACAAAAAAATGGGGAGG
AAGCGGAAGCACAGTGAGACGGAAGCTCCGGCGCCGGTGAAAAAGAGCGATGAGCCTGCT
CCGGATAGACCCAAAAGGACTCTATTGGGATGGAAAGATAAGAGTGAAGGTGAAGCTGAA
AAAGCTAAAA

Last Updated on 06.12.2018 16:11