Sequences from confirmation amplicon


Confirmation sequences for line 314G09 and hit At1g60460 are shown.
Line ID 314G09
Locus At1g60460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22276659
Primers used for PCR FV58 - 8474, show primer details
   
Sequence Name 41-K100896-0022-314-G09C-8409
GenBank Accession LN504005 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K100896-0022-314-G09C-8409
CAATTGACTGAAAACACACAATCCGAATATATAATCAAGTCAAGGGCACTCACAGTGGCT
TAGAAATCAGTGAAATAAGATATAAATTGAGGAACTTGCACACCTAAACTTTGCACCATT
CTTAGCCTGAGAAGGTTGTCTTTTCAGTCTTTTGTTCGCTATATACTCATCCAGATTTAT
ATGATAATAGTATACCTCATCGTCAGTAAAGCCTGCACTTCCATCACCACAGTGGAGGAA
GAACAATAACAAGTTACTTAGTTTCGGTGACTCGAACTTAATGTAGACAACTAACAATCA
ATCATATAACCCAGAGAGGAAAACAAGATATGTTTCTCCATTGGACTTACAAGCAAACCA
AGGGAAAA
   
Sequence Name 41-K100904-0022-314-G09C-FV58
GenBank Accession LN504006 [GenBank]
Sequencing primer gene specific primer, FV58: 5'-TTTTTCCTGGTTTTGCTTGTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K100904-0022-314-G09C-FV58
GGTTATATGATTGATTGTTAGTTGTCTACATTAAGTTCGAGTCACCGAAACTAAGTAACT
TGTTATTGTTCTTCCTCCACTGTGGTGATGGAAGTGCAGGCTTTACTGACGATGAGGTAT
ACTATTATCATATAAATCTGGATGAGTATATAGCGAACAAAAGACTGAAAAGACAACCTT
CTCAGGCTAAGAATGGTGCAAAGTTTAGGTGTGCAAGTTCCTCAATTTATATCTTATTTC
ACTGATTTCTAAGCCACTGTGAGTGCCCTTGACTTGATTATATATTCGGATTGTGTTGTT
TTCAGTCAATTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTTC
C
Primers used for PCR FV59 - 8474, show primer details
   
Sequence Name 42-K100896-0022-314-G09C-OriL
GenBank Accession LN504392 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K100896-0022-314-G09C-OriL
TTACAATTGAATCAATTGGCTTCATAATTGGCTTCATGTCAAAAGGTCATTTACTCTTTC
TTTTGAGTGTTTTTGGAAGTATGGATGTTCTCGTGGCACCGATAATTGGCTTCTTTCAAA
AGGTCTTCCCTTCTCTTCTGCAACCTTGTTGCTCTTGTAACTACTTATTTCAATTGGATT
CAAGTACAGAACACAACGTTACAAAAACACTGCTAACCACTTGTTATAGAACCAAGATAA
AGAGCCAAAATATTACATCTTTAAGGGAAGAAGAACCGTACTGAATTCCAATGGAATGAA
AGTTTATGTTTGAAGGTCGCTCATTAGTTAACCAGTGTTTAAGCTACTGCTTTAATTTGG
TCAAGCAATGAATCTGTATCATGAATAACCGAAGCTTGTTTAGTGGTTTATATTTAGCAT
ATCTTTTAAATTGGTATTGTTGGCCTTCTGGCCCTATCATTTGAGTGTAGTCAATTTTGT
CAACTTAGTTCCCTCATGATTGCTTAAGTTACACTGTAATGATATTTGTATCCTGTTTTC
TTGCAGATCATTGTTCTACAGATACTTGTAAGTTCAAGTGACTCCCTTTGGTGATTTACA
ATTTTGTCTAGAAAATTGGTTTCCCTATACATGGTAATTTAGAAAGAGAACCAAAC
   
Sequence Name 42-K100904-0022-314-G09C-FV59
GenBank Accession LN504393 [GenBank]
Sequencing primer gene specific primer, FV59: 5'-AATTCCATGATTTGTTTGGTTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K100904-0022-314-G09C-FV59
GACAATTTTCTGACAAATTGTAAATCACCAAAGGGAGTCACTTGAACTTACAAGTATCTG
TAGAACAATGATCTGCAAGAAAACAGGATACAAATATCATTACAGTGTAACTTAAGCAAT
CATGAGGGAACTAAGTTGACAAAATTGACTACACTCAAATGATAGGGCCAGAAGGCCAAC
AATACCAATTTAAAAGATATGCTAAATATAAACCACTAAACAAGCTTCGGTTATTCATGA
TACAGATTCATTGCTTGACCAAATTAAAGCAGTAGCTTAAACACTGGTTAACTAATGAGC
GACCTTCAAACATAAACTTTCATTCCATTGGAATTCAGTACGGTTCTTCTTCCCTTAAAG
ATGTAATATTTTGGCTCTTTATCTTGGTTCTATAACAAGTGGTTAGCAGTGTTTTTGTAA
CGTTGTGTTCTGTACTTGAATCCAATTGAAATAAGTAGTTACAAGAGCAACAAGGTTGCA
GAAGAGAAGGGAAGACCTTTTGAAAGAAGCCNATTATCGGTGCCACGAGAACATCCNTAC
NTCCNAAAACACTCANAAGAAAGAGTAAATGACCTTTTGACATGAAGCCNATTATGAAGC
CNATTGATTCANTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAATG
TAATT

Last Updated on 06.12.2018 16:11