Sequences from confirmation amplicon


Confirmation sequences for line 150A08 and hit At1g63630 are shown.
Line ID 150A08
Locus At1g63630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23587540
Primers used for PCR 20op - 8474, show primer details
   
Sequence Name 34-K115075-0022-150-A08C-20op
GenBank Accession LN501527 [GenBank]
Sequencing primer gene specific primer, 20op: 5'-GGATGTAGACCCAATGTTGAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115075-0022-150-A08C-20op
ATGGTTGAAACTGGATGTAGACCCAATGTTGTCACCTTCAACACACTGATGAACAGTCTT
TGTCGTGAGGGTCGAGTTCTTGAAGCGCCTGCTCTTGTTGATCGGATGGTAGAAGAAGGT
CATCAACCTAACGCGGTTACTTACGGAACAATTGTAAATGGATTGTGTAAGATTGGTTGT
CTCGGCTTTGAATCTGCTTAGGAAGATGGGGCAAAGCCACATCAAAGCCCATGTTGTAAT
CTCTACTGCCATCGTTGATCGCCTTTGCAAGGACGGGAACCACATCAATGCTCAAAACCT
TTTCACTGAAATGCATGAGAAAGGTATTTTTCCCAATGTTCTCACTTACAATTGTATGAT
AGACTCTTTTTGTCACTCTGGTAGATGGAGTGATGCCGACCAATTGTTGCGTCATATGAT
TGAAAAGCAAATCACTCCTGATATTGTTACTATCAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGT
   
Sequence Name 02-K115074-0022-150-A08C-8409
GenBank Accession LN487155 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115074-0022-150-A08C-8409
AACAATATCAGGGATTGATTTGCTTTTCAATCATATGACGCAACAATTGGTCGGCATCAC
TCCATCTACCAGAGTGACAAAGAGAGTCTATCATACAATTGTAAGTGAGAACATTGGGAA
AAATACCTTTCTCATGCATTTCAGTGAAAAGGTTTTGAGCATTGATGTGGTTCCCGTCCT
TGCAAAGGCGATCAACGATCGCAGTACAGATTACGACAACGGCTTTGATGTGGCTTTGCC
CCATCTTCCTAAGCAGATTCGAAGCCGAGACAACCAATCTTACACAATCCATTTACAATT
GTTCCTTAAGTAACCGCGTTAGGTTGATGACCTTCTGCTACCATCCGATCAACAAGAGCT
AGCGCTTCAAGAACTCGACCCTCAC

Last Updated on 06.12.2018 16:11