Sequences from confirmation amplicon


Confirmation sequences for line 361C10 and hit At1g64220 are shown.
Line ID 361C10
Locus At1g64220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23833005
Primers used for PCR KF47 - 8474, show primer details
   
Sequence Name 84-K118656-0022-361-C10C-KF47
GenBank Accession LN514479 [GenBank]
Sequencing primer gene specific primer, KF47: 5'-GCATGAACTCAGATCCTAAACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K118656-0022-361-C10C-KF47
GTTTATCACAAATTGGATCTTCTTCGTATGTTCTTCTTAGATGCTTTTACTTTTTTTCCC
AAATTTGTTCTGTTTTCTTCGTTTTATGAAATGGATTTATATTTGTTTCTGATTCTATAA
TTCAAAGTCTCATTCTTTATTGCATGATTCAAATTCAATAAAATCAAATGATCTATGAAG
AAACAAAGAATATTGCTTTAATGGTTACTACTACTAATTTGTGTTGTCTCACTCTGCATC
AACTGATAGAATATCGTCAATCAATTTCAAAACTAGAGCTATATGAGGTCTTTCTTGTTG
CTTCACAGCAACACATGCCAATCCTATTTGCAACATCTCAACCATCTCCGCCTCGAAACC
ACCTGATTGACTCAATATCTCCGTATCATACACTTCTCCAGACCACTCTTAAGGCACCAC
AGATCTCATCC
   
Sequence Name 58-K118655-0022-361-C10C-8409
GenBank Accession LN509837 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118655-0022-361-C10C-8409
ATTTCGAAGAGGCTTCCATGATTGTATTAGCTATAAACCGCGAGCTTATCATCTTAGGAG
TAGTAGTAAGCCTTTAGCTCAGCTACATTTTCATGACTGATCGTACCAATGATCTCCATT
TGTTGCTCAAACTCTCATCTTCCAACGACAACTTCCTTCAATCGTTTTACAACGACCGTG
CTCATGTCCTCCATTGTTACCTTATAGGTTGTACCAAAAGCACCCTTCCCCAAAACTTCT
AAAACGAAGAAAACAGAACAAATTTGGGAAAAAAAGTAAAAGCATCTAAGAAGAACATTA
CGAAGAAGATCCAATTTGTGGATTAAACGGGAGAGAGGAGGTGAAAAAGATGAGGTTTAG
GATCTGA

Last Updated on 06.12.2018 16:11