Sequences from confirmation amplicon


Confirmation sequences for line 568A01 and hit At1g66440 are shown.
Line ID 568A01
Locus At1g66440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24783541
Primers used for PCR 44xr - 8474, show primer details
   
Sequence Name 47-K119480-0022-568-A01C-44xr
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 44xr: 5'-TTAGCACTTAAACTCGTTACCCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K119480-0022-568-A01C-44xr
ATTCTATCCGCTTTGAAAAATACCTATAGTTTTTGGGTTCGGTAAAAGGTATTATAATTT
TTTTTTTCCTGGTAATTAAGGATTAGGTATCATATATCAAATATATTTTGAACTTCCCGT
TCTGTACCCTCACTATTATCCGATCCATAGTAGTTTTAACTTATCTAGATAAGGTGTTAT
TCAGTATATTGTTACATATAATCCTATATAAAATTTGATTATTCAGGTATTTAAGGTTTT
AAAAGTATAAATATTGACTAAAATGTAAATATTTGAATGTAGTTGTGGGTATAAAGTTGG
GATTAATTAACAGTACATATATTACCTGTAAAGATCGGATTGGATAACAAATAAATATTT
CGCAAGATATGAGATAATTTCGCAAGAAAATGACACAAATAAATATTTTGCTTTGCATCA
TCGGTGATTCTTGAACATACAAATTCAATAAGAGCTAACGATAAAGCTCCAAACTAAAAA
CCTTCGACCTCATACAACCGGTGGAACAAAAGTTTAATGTAAAACGTCGGAACACTATTT
TGTGTGGACAACGATCTTTACATTCCCTACAAATAGGTCTAGTCATACAATGGTTGTTGC
GAAAAACACGTACCTTTCTACCTTTGAATAACCAAAATGAACCGGGCTTCATATATAAGT
CCTTCCCAATCAAACATTCAATGTGTAGGGTGACACAACCATACTCATCACAATTATAAA
ACTGCCCTTTCGGATTTATTTTTCTCTCGCAAGCTTCACACCAATACGCCATGGTACTTG
TCTCGATCTCCTTCCCATAAGAAAGAATGAGCACATGATTATCATGCTTATACCT
   
Sequence Name 23-K119479-0022-568-A01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K119479-0022-568-A01C-8409
CATTTACAATTGAATCTATTTTGGTAAATTCTACTCTTGTATGGAATGCAATTTCATTCT
CCACGAAGAATGTGCAAATTTTTCTCGCAAAATACAACACCCGATACATCCACATCTGCT
CACTTTAGTGACTTCCGCGACCGATAAAGCCAACACTCATGATTTATGTTCAGCTTGCCC
ATGGTTGTTCACCACCGGTTTCTTCTATGAGTGCCACAGAGAAGGATGTCGTTTTAATCT
ACATGTGCGGTGCGGGACAATTTCTGAACCATTAGTTCATGGAAGTCATAGGCATCCTTT
ATTCCTAACATCTAAACCAGGAGATTGGAGAACATGTAGTGTTTGCAAAGAGTCAAGCCG
TTATGTCACACATGAAACATTTAATTGCGTTCAATGTGTCTTTGCTTTGTGTTTCAAGTG
TGCTGCTATACCTCAGAAGGTGAGGTATAAGCATGATAATCATGTGCTCATTCTTTCTTA
TGGGAAGGAGATCGAGACAAGTACCATGGCGTATTGGTGTGAAGCTTGCGAGAGAAAAAT
AAATCCGAAAGGGCAGTTTTATAATTGTGATGAGTATGGTTGTGTCACCCTACACATTGA
ATGTTTGATTGGGAAGGACTTATATATGAAGCCCGGTTCATCTTGGTTATTCAAAGGTAG
AAAGGTACGTGTTCTTCGCAACAACCATCGTATGACTAGACCTATTTGTAGGGAATGTAA
AGATCGTTGTCCACACAAAATAGTGTTCCGACGTTCTACATTAAACTTTTGTTCCACCGG
TTGTATGAGGTCGAAGGTTTTTAGTCTGGAGCTTTATCGTTAGCTCTTATTGAATTTGTA
TGTTCAAGAATCACCGATGATGCAAAGCAAAATATTTATTTGTGTCATTTTCTTGCGAAA
TTATCTC

Last Updated on 06.12.2018 16:11