Sequences from confirmation amplicon


Confirmation sequences for line 266D11 and hit At1g66470 are shown.
Line ID 266D11
Locus At1g66470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24796570
Primers used for PCR 18nz - 8474, show primer details
   
Sequence Name 65-K114716-0022-266-D11C-18nz
GenBank Accession LN511457 [GenBank]
Sequencing primer gene specific primer, 18nz: 5'-CATTAAAATAAAACTCAATCTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K114716-0022-266-D11C-18nz
TTATTTTGTTCGAACGAAGAGAAAGATAAGTGAACGTCTCAAGATATTGCAAGAACTTGT
TCCCAATGGCACCAAGGTACACATCCATATAACCATTTATGACAGATTTATTATATTACT
TATTCTCATGATAATAATATTTGTTATGTTTCACAGGTTGATTTGGTGACAATGCTTGAA
AAGGCTATTAGTTATGTCAAGTTCCTTCAAGTACAAGTTAAGGTATATTAGTTATGACAA
ACATCCCTCAAATTAATTTTCAGGATTGATTTAATTACCATATTTATCGAATTATTACGT
AACACGTATTGATATAATCTATTGTTTGTTAGGTATTAGCGACCGATGAGTTTTGGCCGG
CTCAAGGAGGAAAAGCTCCTGACATTTCTCAAGTTAAAGACGCCNTTGATGCCATTCTCT
CCTCATCACAACGAGACAGGGTTATCATTTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATTTCCGCACA
   
Sequence Name 17-K114715-0022-266-D11C-8409
GenBank Accession LN493961 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114715-0022-266-D11C-8409
ATGATAACCCTNTCTCGTTGTGATGAGGAGAGAATGGCATCAATGGCGTCTTTAACTTGA
GAAATGTCAGGAGCTTTTCCTCCTTGAGCCGGCCAAAACTCATCGGTCGCTAATACCTAA
CAAACAATAGATTATATCAATACGTGTTACGTAATAATTCGATAAATATGGTAATTAAAT
CAATCCTGAAAATTAATTTGAGGGATGTTTGTCATAACTAATATACCTTAACTTGTACTT
GAAGGAACTTGACATAACTAATAGCCTTTTCAAGCATTGTCACCAAATCAACCTGTGAAA
CATAACAAATATTATTATCATGAGAATAAGTAATATAATAAATCTGTCATAAATGGTTAT
ATGGATGTGTACCTTGGTGCCATTGGGAACAAGTTCTTGCAATATCTTGAGACGTTCACT
TATCCTTTCTCTTCGATTCTGAACAAAATAAAACATATGTAAATATGTGTTGAGAGATTG
AGTTTTATTTTAATG

Last Updated on 06.12.2018 16:11