Sequences from confirmation amplicon


Confirmation sequences for line 210H03 and hit At1g66500 are shown.
Line ID 210H03
Locus At1g66500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24811761
Primers used for PCR 11us - 8474, show primer details
   
Sequence Name 45-K117737-0022-210-H03C-11us
GenBank Accession LN505670 [GenBank]
Sequencing primer gene specific primer, 11us: 5'-TCTAAAATTCTCCTCTCTCAACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K117737-0022-210-H03C-11us
GGTTCTTTTCCTTTCTGCGATCGATCTCTGAAAACTCATAACTGTTGTTTAATCGTGAAG
ATGGCATCAAACGGATCTTTCTCTGCTCAGAGAAACGCAAACGCTAGAACAACGATGAAG
AGAAGAAGCGACAATCGCGGTTACGGTGGCGGAATCGGAGGTTATCAAGAGGAGACGAAC
CGTTACGCGCCGCCGCAAAAACGTTTCAGATCACAAGCGCAACAGCAATTCCGATCTGGT
CATAATCCTCTGTATCATCATCATGGTAGTAATAATAATAACGTGTCTAGGGTTTCTTCT
CAATCATATAACAATTGCGGTGTTGATGTGATTGCTTCTAATTCAAGTTTTGCATTACGT
AACAATGATTCTAACACCAACAATTATCAGAAACCCTTTGTTGCTGGTTATGGAAACCCT
AATCCCCAAATTGTTCCTCTTCCATTACCTTATCGTAAGCTTGATGACAATCTCTCTTTA
GATTCGTTACCTGATTGGGTTCCGAATTCAAGAACCCTAACTCCGAATTATCCTGTTAGG
TCATCGAATTTTGTACCAAACACTCCTGTTTTTACGAATGTTCAAAATCCGATGAATCAT
TCGAACATGGTTTCAGTTGTGTCTCAATCTATGCATCAACCAATTGTTTTGAGCAAAGAA
TTGACTGATTTGCTTAGTCTTCTTAACAATGAGAAAGAGAAGAAAACTTTAGAAGCAAGT
AATAGTGATTCTTTGCCTGTTGGATTAAGCTTTGATAATCCATCGTCACTTAATGTTCGA
CATGAATCTGTGATTAAGTCTCTGTATTCTTGACAGGATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTCAATTCAAAATGT
   
Sequence Name 13-K117736-0022-210-H03C-8409
GenBank Accession LN492233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117736-0022-210-H03C-8409
ATTTCATTGAATTATCCTGTCAGAATACAGAGACTTAATCACAGATTCATGTCGAACATT
AAGTGACGATGGATTATCAAAGCTTAATCCAACAGGCAAAGAATCACTATTACTTGCTTC
TAAAGTTTTCTTCTCTTTCTCATTGTTAAGAAGACTAAGCAAATCAGTCAATTCTTTGCT
CAAAACAATTGGTTGATGCATAGATTGAGACACAACTGAAACCATGTTCGAATGATTCAT
CGGATTTTGAACATTCGTAAAAACAGGAGTGTTTGGTACAAAATTCGATGACCTAACAGG
ATAATTCGGAGTTAGGGTTCTTGAATTCGGAACCCAATCAGGTAACGAATCTAAAGAGAG
ATTGTCATCAAGCTTACGATAAGGTAATGGAAGAGGAACAATTTGGGGATTAGGGTTTCC
ATAACCAGCAACAAAGGGTTTCTGATAATTGTTGGTGTTAGAATCATTGTTACGTAATGC
AAAACTTGAATTAGAAGCAATCACATCAACACCGCAATTGTTATATGATTGAGAAGAAAC
CCTAGACACGTTATTATTATTACTACCATGATGATGATACAGAGGATTATGACCAGATCG
GAATTGCTGTTGCGCTTGTGATCTGAAACGTTTTTGCGGCGGCGCGTAACGGTTCGTCTC
CTCTTGATAACCTCCGATTCCGCCACCGTAACCGCGATTGTCGCTTCTTCTCTTCATCGT
TGTTCTAGCGTTTGCGTTTCTCTGAGCAGAGAAAGATCCGTTTGATGCCATCTTCACGAT
TAAACAACAGTTATGAGTTTTCAGAGATCGATCGC

Last Updated on 06.12.2018 16:11