Sequences from confirmation amplicon


Confirmation sequences for line 172D10 and hit At1g69940 are shown.
Line ID 172D10
Locus At1g69940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26344827
Primers used for PCR 67pm - 8474, show primer details
   
Sequence Name 19-K115349-0022-172-D10C-67pm
GenBank Accession LN494889 [GenBank]
Sequencing primer gene specific primer, 67pm: 5'-CAGTTGTAGAAAGCAGCAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K115349-0022-172-D10C-67pm
TCCTTAGTTTTCCATCTGGTGCCGGCGCAGTGTTCTACATATGCAAAACAATAAATCACT
CGGCCATGAAATAGTCGACTAGTCACATAGATGTATGATATATAGTAGGATCACTTACCT
TAACGACGATGTTAACGGCCATGAAATAGTCGGATAAGATAATGAGAGAGGCGCTATCAA
CGGTTCCATACTTGGCGGCGGTACCGTCATAGGTGATAACAGGCATGGCATTGGGTTGTC
CCATCAATGTAATGAAGGGTTTGTTCCTGTCGATAGTGACCTTCTCTTTGTATTCACCAG
GAGCCATCTTTATGATCACCCGCTTTGTGTTCCCTGCAGGAACGCTCTTTATTGCGTCCG
TTAGTGTTTTGAATTCACCACCCTTTGGATTCACCTGCAATAATTTTTACATGTTTTTCA
AAAAAATTGTATCAAAATCTATTGGTACAAAGGATGAGTTTAAGACAATAAGTGTGAATA
GTACGTTGATGATACGTGGAGCAGCCTCAGCAGCTACAAGAGCAGGATCTAAGCCTTTAC
GTTGTAACCGTAAACAAATGAATATATCCTCTTTCTTTTCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 03-K115348-0022-172-D10C-oriL
GenBank Accession LN487613 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K115348-0022-172-D10C-oriL
CATTTCATTGAATATATCCTGAAAGAAAGAGGATATATTCATTTGTTTACGGTTACAACG
TAAAGGCTTAGATCCTGCTCTTGTAGCTGCTGAGGCTGCTCCACGTATCATCAACGTACT
ATTCACACTTATTGTCTTAAACTCATCCTTTGTACCAATAGATTTTGATACAATTTTTTT
GAAAAACATGTAAAAATTATTGCAGGTGAATCCAAAGGGTGGTGAATTCAAAACACTAAC
GGACGCAATAAAGAGCGTTCCTGCAGGGAACACAAAGCGGGTGATCATAAAGATGGCTCC
TGGTGAATACAAAGAGAAGGTCACTATCGACAGGAACAAACCCTTCATTACATTGATGGG
ACAACCCAATGCCATGCCTGTTATCACCTATGACGGTACCGCCGCCAAGTATGGAACCGT
TGATAGCGCCTCTCTCATTATCTTATCCGACTATTTCATGGCCGTTAACATCGTCGTTAA
GGTAAGTGATCCTACTATATATCATACATCTATGTGACTAGTCGACTATTTCATGGCCGA
GTGATTTATTTTTTTGCATATGTAGAACACTGCGCCGGCACCAGATGGTAAAACTAAGGG
AGCACAAGCTTTATCCATGAGAATCTCCGGAAATTTTGCTGCTATCTACAACTG

Last Updated on 06.12.2018 16:11