Sequences from confirmation amplicon


Confirmation sequences for line 285C08 and hit At2g03220 are shown.
Line ID 285C08
Locus At2g03220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:970666
Primers used for PCR 06ow - 8474, show primer details
   
Sequence Name 48-K115005-0022-285-C08C-06ow
GenBank Accession LN506589 [GenBank]
Sequencing primer gene specific primer, 06ow: 5'-TCAATGGCTCTGGAAAGAGATAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K115005-0022-285-C08C-06ow
ACCTAGATCTAACAAAATGGAAAAAGAATAAATCTGATCTCTCCCTTTTACTTTCCTCAT
TTGTTACATAGGAAGAGTAAGAGAATAAGCAAAAGATCATCAAAAAAAAACTGAGACTTT
GGTAATAAAAACTCAACCTTTATGCTCTTGTTTTATCTATTTTAAGAGAAAATGTCAAAA
GCACAAAAATTGACTTTATTAAAGCTAATTTAAGAAGTTACATGATACATCAAATAATCA
AAAACAGAGTAAGGCACACAAAAAATCATACTAGCTTAAGTCCCCAGCTGATATCCTCAC
AATGTCTCACATGAGGAACTAGTGTTCCCGTGTCAATACCCGTTTTCGCTTTACAATCAT
AGAATGGAGGCGAGTGGAAACAAGGCTCCATCGACATAGCCCGACCACACGAAGGATCGG
GAGTTGTACGGTTTTCGGGTCTATAGAGTATCCAAGGCTTTAAACCTCCAAGACCTTGAG
CTACATATCCAAATGTAGACCAAGCACTTGTCACAAGATTATCTGTCAAACATGGTTGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCA
   
Sequence Name 16-K115004-0022-285-C08C-8409
GenBank Accession LN493546 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115004-0022-285-C08C-8409
TCGGAATGAGCATTTCATTGAATATATCCTGCACCATGTTTGACAGATAATCTTGTGACA
AGTGCTTGGTCTACATTTGGATATGTAGCTCAAGGTCTTGGAGGTTTAAAGCCTTGGATA
CTCTATAGACCCGAAAACCGTACAACTCCCGATCCTTCGTGTGGTCGGGCTATGTCGATG
GAGCCTTGTTTCCACTCGCCTCCATTCTATGATTGTAAAGCGAAAACGGGTATTGACACG
GGAACACTAGTTCCTCATGTGAGACATTGTGAGGATATCAGCTGGGGACTTAAGCTAGTA
TGATTTTTTGTGTGCCTTACTCTGTTTTTGATTATTTGATGTATCATGTAACTTCTTAAA
TTAGCTTTAATAAAGTCAATTTTTGTGCTTTTGACATTTTCTCTTAAAATAGATAAAACA
AGAGCATAAAGGTTGAGTTTTTATTACCAAAGTCTCAGTTTTTTTTTGATGATCTTTTGC
TTATTCTCTTACTCTTCCTATGTAACAAATGAGGAAAGTAAAAGGGAGAGATCAGATTTG
ATTTCTTTTTCCATTTTTGTTTAGATCTTAGGGTTTCTGAATAATGTATCCCTCCAGAAG
AAGATTATCTCTTTC

Last Updated on 06.12.2018 16:11