Sequences from confirmation amplicon


Confirmation sequences for line 522D04 and hit At2g27020 are shown.
Line ID 522D04
Locus At2g27020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11530911
Primers used for PCR CW75 - 8474, show primer details
   
Sequence Name 20-K104153-0022-522-D04C-CW75
GenBank Accession LN495188 [GenBank]
Sequencing primer gene specific primer, CW75: 5'-GGTCACCTAATGGGTAGTTAAGCA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K104153-0022-522-D04C-CW75
GATCCAGTTTTCGCAAAACTGTCATACTTTTTTGATCGCCACAACTATGTCTCTCAGTTT
GGCTAGAGATGTAGTAAAAAACAAACTTATTGTATCCATCACAATTATAGGTAATTTACT
GTCTTGGTTTTAAAAACATGTAATGTTGAGCATCCCAAAGTTTGTGTGTCCCAATGCTAG
AGCATATTATAGTCTTACCTCTAGTGAGTTCATAAAGAAGTGTTGTATGATCATATATTT
TGTTGAGTAAGAGAAATATTTCTTTCTTCCTCATGTGGTTGATTATTCAAAATTATACAA
AAGAGGAAAATCACTTAAAAATATTCACGATACCGTTAAAAGATTACCAAAGAATGGGCC
AAATCATAATATTAATAAAGCCCGTTAATAACCGAATCGACCCACCCGACAAAACAACCC
GAATCGTTTTAATATCCAACCCGGAGTCAGACGGAGCTCCNAAGATTTGGCTTGTCTCTG
GCTTGAGAACAAAGAAAACCCCTTTCTTCGATCTCAGCTTAAATCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCG
   
Sequence Name 20-K104154-0022-522-D04C-8409
GenBank Accession LN495189 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K104154-0022-522-D04C-8409
GAGCCTTTACAATTGATTTAGCTGAGATCGAAGAAAGGGGTTTTCTTTGTTCTCAAGCCA
GAGACAAGCCAAATCTTTGGAGCTCCGTCTGACTCCGGGTTGGATATTAAAACGATTCGG
GTTGTTTTGTCGGGTGGGTCGATTCGGTTATTAACGGGCTTTATTAATATTATGATTTGG
CCCATTCTTTGGTAATCTTTTAACGGTATCGTGAATATTTTTAAGTGATTTTCCTCTTTT
GTATAATTTTGAATAATCAACCACATGAGGAAGAAAGAAATATTTCTCTTACTCAACAAA
ATATATGATCATACAACACTTCTTTATGAACTCACTAGAGGTAAGACTATAATATGCTCT
AGCATTGGGACACACAAACTTTGGGATGCTCAACATTACATGTTTTTAAAACCAAGACAG
TAAATTACCTATAATTGTGATGGATACAATAAGTTTGTTTTTTACTACATCTCTNGCCAA
ACTGAGAGACATAGTTGTGGCGATCAAAAAAGTATGACAGTTTTGCGAAAACTGGATCTT
GTTTAATATCAAATCTTTAAGTGAAGGTCATGATGCTTAACTACCC

Last Updated on 06.12.2018 16:11