Sequences from confirmation amplicon


Confirmation sequences for line 894E02 and hit At2g35210 are shown.
Line ID 894E02
Locus At2g35210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14836650
Primers used for PCR 29kk - 8474, show primer details
   
Sequence Name 69-K113845-0022-894-E02C-29kk
GenBank Accession LN512249 [GenBank]
Sequencing primer gene specific primer, 29kk: 5'-GACTTGAGGGCTCATGTAATCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K113845-0022-894-E02C-29kk
CGCTCGTATAATAACAGATTCCTCTGGCTTTTGATCATAAAGAGTCCCACTCGACTGCAA
GAGGAAAAAATGTGAGCTCTCATTACAAATTCTATAAAGAGGAAAGAACATTACTGACCT
TAGTAGTTAGCTTCCGGGCGCCAAGTCCGCCAGTTTTGCCTGTCTTCTTAGCACCAAGAG
GTTTCTTAACGCTGCGAGAAATTCTTGGTGAGACGGGAACAACATCAGGCTTTTCTTGCT
GCTTTAGAGTATTACTCTCTTTAAGAGCTTCACTGGTTTTGATGCTGGAGAGTCCATTAG
GCACTTGTGTTGAATCAGGAGGAGATGGAGGCAAATCCAATTCTTCCTCAGCCTTGCTTT
TAGCAACTTCTTTAGCAAGTATCTGTTTATACAAATCAGCAGCTCTTGAGGTATACTTAG
CCTCGGTTTTACCTCCATCGCTCCATCCGTACTGTTTGAAAAAGACTTGTGCCCTGTTGT
TTCCTCCATATATCATCATTTTCAATTGCTCTGAGCTCCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 42-K113844-0022-894-E02C-8409
GenBank Accession LN504536 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113844-0022-894-E02C-8409
CTCAGAGCAATTGAAAAGATGATATATGGAGGAAACACAGGGCACAAGTCTTTTTCAAAC
AGTACGGATGGAGCGATGGAGGTAAAACCGAGGCTAAGTATACCTCAAGAGCTGCTGATT
TGTATAAACAGATACTTGCTAAAGAAGTTGCTAAAAGCAAGGCTGAGGAAGAATTGGATT
TGCCTCCATCTCCTCCTGATTCAACACAAGTGCCTAATGGACTCTCCAGCATCAAAACCA
GTGAAGCTCTTAAAGAGAGTAATACTCTAAAGCAGCAAGAAAAGCCTGATGTTGTTCCCG
TCTCACCAAGAATTTCTCGCAGCGTTAAGAAACCTCTTGGTGCTAAGAAGACAGGCAAAA
CTGGCGGACTTGGCGCCCGGAAGCTAACTACTAAGGTCAGTAATGTTCTTTCCTCTTTAT
AGAATTTGTAATGAGAGCTCACATTTTTTCCTCTTGCAGTCGAGTGGGACTCTTTATGAT
CAAAAGCCAGAGGAATCTGTTATTATACAAGCGACATCACCAGTTTCAGCTAAATCAGCC
AGGTCATCGTTTTCCTCTCGTTTTGATTATGCGGACAATGTTCAAAACAGGGAAGATTAC
ATGACCC

Last Updated on 06.12.2018 16:11