Sequences from confirmation amplicon


Confirmation sequences for line 704H08 and hit At2g36850 are shown.
Line ID 704H08
Locus At2g36850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15457605
Primers used for PCR KG48 - 8474, show primer details
   
Sequence Name 86-K038033-022-704-H08C-KG48
GenBank Accession LN514619 [GenBank]
Sequencing primer gene specific primer, KG48: 5'-TTTTACATTGGTGGCAAATTGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K038033-022-704-H08C-KG48
GCGTGGTTCATGTCGAATCAAGAGACCAGTTTTGTAACGTTGGGTCAACGGGTTCTAGCA
TATCCACTTAAGTGAGTATGCTCGCTTAAGACTATAACTTGTGCAATAGGACTACATTTC
ATTGTGAACTTTCCTTTATTGTGGAGCTTGTTACATGTTTTAGCTTTCTCTTCATCTGCT
TGCATTTACTTGGTAAAATGGGTTCAGCACTCGTTTTCCGGTTTATTGGATTGTTTTTAT
TTAATCTATTACCACTTTCCAGTGACAGCTTAGTTTCAATTTGTTCAAAACTAATCTTGC
AGAGTTCGAATGCACTATGGGCATCCAGATGTGTTCGATAGAATATTTCATATAACTCGC
GGAGGAATCAGCAAAGCATCCCGTGTTATTAACATCAGTGAAGATATATATGCTGGTATG
CGTAGTAGATTGGTGCTATATATATTTAAATTCCTGATTGCTGTTTTAAACTTAATAAGT
TCTTTTTGAACTGTCAACGTTCTAATTTTTAAAAAAGCTTAACCATGATATGTTCTTGAA
AAATCTGAGCTTTTTACCTATAAACAATGACAATATTTGCAGGATTTAACTCGACACTCA
GGCAGGGGAATATCACCCACCATGAGTATATTCAGGCAACGACTTACTTGCTTTAAATGT
TTCTTCACTTAGCTTGTATCTCATGTCGATCTGAATACATGATCCATACAAATTCTTTCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCATAAACAAATTATTCTAA
   
Sequence Name 30-K038033-022-704-H08C-8409
GenBank Accession LN499630 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K038033-022-704-H08C-8409
TCCGGACTGAGCCATTTCAATTGAATATATCCTGAAAGAATTTGTATGGATCATGTATTC
AGATCGACATGAGATACAAGCTAAGTGAAGAAACATTTAAAGCAAGTAAGTCGTTGCCTG
AATATACTCATGGTGGGTGATATTCCCCTGCCTGAGTGTCGAGTTAAATCCTGCAAATAT
TGTCATTGTTTATAGGTAAAAAGCTCAGATTTTTCAAGAACATATCATGGTTAAGCTTTT
TTAAAAATTAGAACGTTGACAGTTCAAAAAGAACTTATTAAGTTTAAAACAGCAATCAGG
AATTTAAATATATATAGCACCAATCTACTACGCATACCAGCATATATATCTTCACTGATG
TTAATAACACGGGATGCTTTGCTGATTCCTCCGCGAGTTATATGAAATATTCTATCGAAC
ACATCTGGATGCCCATAGTGCATTCGAACTCTGCAAGATTAGTTTTGAACAAATTGAAAC
TAAGCTGTCACTGGAAAGTGGTAATAGATTAAATAAAAACAATCCAATAAACCGGAAAAC
GAGTGCTGAACCCATTTTACCAAGTAAATGCAAGCAGATGAAGAGAAAGCTAAAACATGT
AACAAGCTCCACAATAAAGGAAAGTTCACAATGAAATGTAGTCCTATTGCACAAGTTATA
GTCTTAAGCGAGCATACTCACTTAAGTGGATATGCTAGAACCCGTTGACCCAACGTTACA
AAACTGGTCTCTTGATTCGACATGAACCACGCCAGTGATGAAACACTGCAATATCATTTG
CCCAAAATTTAAAAAAA

Last Updated on 06.12.2018 16:11