Sequences from confirmation amplicon


Confirmation sequences for line 770G09 and hit At3g01310 are shown.
Line ID 770G09
Locus At3g01310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:100131
Primers used for PCR JE35 - 8474, show primer details
   
Sequence Name 80-K114263-0022-770-G09C-JE35
GenBank Accession LN513977 [GenBank]
Sequencing primer gene specific primer, JE35: 5'-AACTTGATCCGCAATATCTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K114263-0022-770-G09C-JE35
GTAGCATTTAGACTGCAGATAGCTGTTTCTGTGCAAATTCTTCAGAGTGATTCAGCGTTA
ACGAAAAATAAGAACTACCTAATTTTTCCTTTTACTAATCAAAATAACTACTTATGTGCA
TCTACAGCATCTTGAGATGTATGGCATCCCAGTTCCTAGGTATGCTTGTGTCAATAGAAA
GGTACCAAATCAAGACCTTCATTATTTTGTCGAGGAAGAAGATTTTGTTGAGGTCCATGG
TGAACGCTTCTGGAAGCCATTTGTGGAAAAGCCTGTCAATGGTGAGCTTGCCTCTGGCTA
TCCTCCTTGTTTTATTTATCTTCTGATTTGCAGTTTTTTTTTTTAAAATTATTATTGGCT
AACTATTGACATTCTTTATACGTGTTTAGGAAATGACCATAGTATAATGATATCTCACAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAAATTTCC
   
Sequence Name 37-K114262-0022-770-G09C-oriL
GenBank Accession LN502700 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K114262-0022-770-G09C-oriL
ATTTACATTGATATATCCTGTGAGATATCATTATACTATGGTCATCTCCTAAACACGTAT
AAAGAATGTCAATAGTTAGCCAATAATAATTTTAAAAAAAAAAACTGCAAATCAAAAGAT
AAATAAAACAAGGAGGATAGCCAGAGGCAAGCTCACCATTGACAGGCTTTTCCACAAATG
GCTTCCAAAAGCGTTCACCATGGACCTCAACAAAATCTTCTTCCTCGACAAAATAATGAA
GGTCTTGATTTGGTACCTTTCTATTGACACAAGCATACCTAGGAACTGGGATGCCATACA
TCTCAAGATGCTGTAAATGCACATAAGTAGTTATTTTGATTAGTAAAAGGAAAAATTAGG
TAGTTCTTATTTTTCGTTAACGCTAAATCACTCTAAAAAATTTGCACAAAAACAGCTATC
TGGCAGTCTAAAATGCTAACCTCAAACACCTTCCGGCAATCATGAAAAAAATTTGCGGAA
TCA

Last Updated on 06.12.2018 16:11