Sequences from confirmation amplicon


Confirmation sequences for line 117A07 and hit At3g04940 are shown.
Line ID 117A07
Locus At3g04940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1367447
Primers used for PCR RH03 - 8474, show primer details
   
Sequence Name 19-K018601-022-117-A07C-RH03
GenBank Accession LN494558 [GenBank]
Sequencing primer gene specific primer, RH03: 5'-TTTTTCCTCTTCCACAATCCTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K018601-022-117-A07C-RH03
CTACTACTAGTAACATGGGTTGAAATCCTACATGGCCATTATATAGTTCTAGATTCATTG
TCAACAAAAGTTTTACAGACTTACACATATATTAGCTAGAAAAAAACCACCAATAGAATG
TCAAAGGACAGTCCACCTTAGAGGCTAGAGATTAGAGATCATACATCATCTGAAATCTTA
AAGTTCATGAAACTATGACAGTGTAAATGTCGAACCGAAATCTTGGTACTTTTTCACCAA
AACGAACCAAGTCAGGTCGTGTAAAAGCCTCATTGAATTGGCAAATTCTCTGCTTCATGT
CTGACTGATTCGAACAATGAGGTCGATAGGTCAGGATATATTCAATTGTAAATGGCTTCA
TGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAA
GAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAA
TGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAA
TAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 20-K018601-022-117-A07C-8409
GenBank Accession LN495013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K018601-022-117-A07C-8409
ATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGACCTATCGACCTCATTGTT
CGAATCAGTCAGACATGAAGCAGAGAATTTGCCAATTCAATGAGGCTTTTACACGACCTG
ACTTGGTTCGTTTTGGTGAAAAAGTACCAAGATTTCGGTTCGACATTTACACTGTCATAG
TTTCATGAACTTTAAGATTTCAGATGATGTATGATCTCTAATCTCTAGCCTCTAAGGTGG
ACTGTCCTTTGACATTCTATTGGTGGTTTTTTTCTAGCTAATATATGTGTAAGTCTGTAA
AACTTTTGTTGACAATGAATCTAGAACTATATAATGGCCATGTAGGATTTCAACCCATGT
TACTAGTAGTAGACAATTTGGCTAGAGGATTGTGGAAAGNGAAAA

Last Updated on 06.12.2018 16:11