Sequences from confirmation amplicon


Confirmation sequences for line 600E08 and hit At3g05580 are shown.
Line ID 600E08
Locus At3g05580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1618343
Primers used for PCR 10xo - 8474, show primer details
   
Sequence Name 41-K119377-0022-600-E08C-10xo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 10xo: 5'-GCAAAAGAGGAGAGGCACTCTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K119377-0022-600-E08C-10xo
GATACCAATAAAGAAAACGACCAAAAGGAGATGAAAATCTAGAGAAGCAGAAAAGAGATG
TTTCTGCAACTATACACAGAATATTTGGACAACACCTTTTGTTTTCTCTCTCTCTCTCTC
TCTTTTGTTTTTTCTACTGATGCTTCTTCTTTGATGACTAAGTAAAGTAGCCTTTTGAGG
TTCTCTTCTCCATTGCAGTTTGCCTACATCTGAAGAAATGCACAGAGCGCTAGCTTGAGC
TTGTGAAACCAAATCTCTCAAGGGAATAAGCTGTATTAAGTTTCAAGACTTCCCCATTTT
CGGTACCTGATCAAAAAGAGAAAATATAGAGAGAAACATGTGTTACATTATGACGTGAAG
TTTCCAAAAGAACAAAACTTGTCATGACTCATCAAGTCCAAAAGGCTTGGCATCAATTGT
AATTACCAATTGTAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGA
   
Sequence Name 17-K119376-0022-600-E08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K119376-0022-600-E08C-8409
CATTGAATATTTACATTGGTAATTACAATTGATGCCAAGCCTTTTGGACTTGATGAGTCA
TGACAAGTTTTGTTCTTTTGGAAACTTCACGTCATAATGTAACACATGTTTCTCTCTATA
TTTTCTCTTTTTGATCAGGTACCGAAAATGGGGAAGTCTTGAAACTTAATACAGCTTATT
CCCTTGAGAGATTTGGTTTCACAAGCTCAAGCTAGCGCTCTGTGCATTTCTTCAGATGTA
GGCAAACTGCAATGGAGAAGAGAACCTCAAAAGGCTACTTTACTTAGTCATCAAAGAAGA
AGCATCAGTAGAAAAAACAAAAGAGAGAGAGAGAGAGAGAAAACAAAAGGTGTTGTCCAA
ATATTCTGTGTATAGTTGCAGAAACATCTCTTTTCTGCTTCTCTAGATTTTCATCTCCTT
TTGGTCTGTTTTCTTTTATTTGGTTATTCTTTTAACACCTTAGTTTAACTTAGAGTGCCT
CTCCTCTTT

Last Updated on 06.12.2018 16:11