Sequences from confirmation amplicon


Confirmation sequences for line 410A07 and hit At3g09630 are shown.
Line ID 410A07
Locus At3g09630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2955365
Primers used for PCR 26gp - 8474, show primer details
   
Sequence Name 28-K112248-0022-410-A07C-26gp
GenBank Accession LN498910 [GenBank]
Sequencing primer gene specific primer, 26gp: 5'-CAATGTGATGTTGAAGTTGAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K112248-0022-410-A07C-26gp
AGAGAGTTAAGGCTAAGAAGGAGAAGCTCGCCAAGAAGAGGAAAACCGTTACCAAGGTAA
TCTCAATACTTGACTTGTTCTCTTATCAACCGTTTTTGGATTGTCTTTGTTTAGTTACTG
ATTACATTTTGATCTGTGGTTGCATTTCATTTGACTTAACTTATATTGCAATTGTTCTAA
TTGACTGAGTTTGTGTATGTTAAGCTATACAATAATCTGTTCCCCTCTGGTTTAGTCTAT
GACTATAGTAGTCTTTCTTTACTAGATCTTACCTTTTCTGTTCTGAATTGTGGTCTAGTA
GTTTGTCTTGTAAATCTGTTGTGTACGATTCTTTGTCTTGATATTGATTTTAGGTTGAGC
TATTAAGCAATTGAATCTCTTAGCACTTTCTTGTCCCTTGTGAAAATTGTTCTTCATATG
TCCTCTTTTCTAACTCCACTTTGAAATATCTATTTTTCTTACATCAGGAGGAGGCGTTGG
CAATCAAAGCAGCAGGCAAGTCGTGGTACAAGAAGCCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATTCC
   
Sequence Name 12-K112247-0022-410-A07C-8409
GenBank Accession LN491642 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K112247-0022-410-A07C-8409
GACTTGCCTGCTGCTTTGATTGCCACGCCTCCTCCTGATGTAAGAAAAATAGATATTTCA
AAGTGGAGTTAGAAAAGAGGACATATGAAGAACAATTTTCACAAGGGACAAGAAAGTGCT
AAGAGATTCAATTGCTTAATAGCTCAACCTAAAATCAATATCAAGACAAAGAATCGTACA
CAACAGATTTACAAGACAAACTACTAGACCACAATTCAGAACAGAAAAGGTAAGATCTAG
TAAAGAAAGACTACTATAGTCATAGACTAAACCAGAGGGGAACAGATTATTGTATAGCTT
AACATACACAAACTCAGTCAATTAGAACAATTGCAATATAAGTTAAGTCAAATGAAATGC
AACCACAGATCAAAATGTAATCAGTAACTAAACAAAGACAATCCAAAAACGGTTGATAAG
AGAACAAGTCAAGTATTGAGATTACCTTGGTAACGGTTTTCCTCTTCTTGGCGAGCTTCT
CCTTCTTAGCCTTAACTCTCTGTGCTTCAGCTAACAGTGACATCCTCTTCGCGGTCTTAG
CATAAGGATTCAA

Last Updated on 06.12.2018 16:11