Sequences from confirmation amplicon


Confirmation sequences for line 770G09 and hit At3g13300 are shown.
Line ID 770G09
Locus At3g13300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4308465
Primers used for PCR 64ad - 8474, show primer details
   
Sequence Name 79-K114263-0022-770-G09C-64ad
GenBank Accession LN513858 [GenBank]
Sequencing primer gene specific primer, 64ad: 5'-AGACTAGAAGTTGCGTTAGGGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K114263-0022-770-G09C-64ad
CTCTAGGGCCCGCATCCAGGAGGAGACTGTTAAGAATGAAAAGGCATTACGTGACCATGC
CCAGCAAATTGTGAATGCAACGACAAACTTCATGAGCAAGGAGTTAAATGCCATGTTTGA
GAAAACGATAAAGAAAGAATTGGCTGCAATTGGTCCAGCCCTAGCACGTTCAGTAGTACC
AGTTATTGAAAAAACTGTATCTTCTGCAATCACAGAGTCCTTTCAGGTAAGATTTTACGA
GAAATTTAGAGCTAATTTTTGAGAAGGTTCATTGTTCTTAAAAGAAAGCTGCTTTCTCTT
TTGTACAGAGAGGAATAGGTGACAAAGCAGTTAATCAGCTTGACAAATCTGTTAATATAA
AGCTTGAAGCAACCGTAGCTAGGCAAATTCAAGCCCAATTTCAGACCTCTGCAGGATATA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 36-K114262-0022-770-G09C-8409
GenBank Accession LN502314 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K114262-0022-770-G09C-8409
TCCTGCAGAGGTCTGAAATTGGGCTTGAATTTGCCTAGCTACGGTTGCTTCAAGCTTTAT
ATTAACAGATTTGTCAAGCTGATTAACTGCTTTGTCACCTATTCCTCTCTGTACAAAAGA
GAAAGCAGCTTTCTTTTAAGAACAATGAACCTTCTCAAAAATTAGCTCTAAATTTCTCGT
AAAATCTTACCTGAAAGGACTCTGTGATTGCAGAAGATACAGTTTTTTCAATAACTGGTA
CTACTGAACGTGCTAGGGCTGGACCAATTGCAGCCAATTCTTTCTTTATCGTTTTCTCAA
ACATGGCATTTAACTCCTTGCTCATGAAGTTTGTCGTTGCATTCACAATTTGCTGGGCAT
GGTCACGTAATGCCTTTTCATTCTTAACAGTCTCCTCCTGGATGCGGGCCCATAGAGCAT
CAGCATTTGACTTGCTGGATTTCTCAATCATTCTCCCTAACGCAACNTCTAGTC

Last Updated on 06.12.2018 16:11