Sequences from confirmation amplicon


Confirmation sequences for line 869H08 and hit At3g13770 are shown.
Line ID 869H08
Locus At3g13770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4519633
Primers used for PCR JF15 - 8474, show primer details
   
Sequence Name 49-K102176-0022-869-H08C-8409
GenBank Accession LN506788 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K102176-0022-869-H08C-8409
GCCATTTACAATTGAATATATCCTGTCATGATCCAACAAATCTTTTAATGGTTTCAACAG
AAAGAAACAAAAGTAACTTTTATGCAAAAGTCTTCAAATAATATGAATAGATGTAACAAC
ATCTAACATACTTGTAGTCTTTAGGTAAAGCATAACATTACATAGCTAAACAGAAGTTGC
ATTAAGACCTACCAGAAGAGCATCTCTAGTTTTAAACAGGTCAAAGAACAAATAGCCATC
TCATGCTGCTCGTAGCCTTTGTATGAAATAAACGTCTGTTGGGACTTGTAGCCTCTGAAG
TAGACGG
   
Sequence Name 49-K102180-0022-869-H08C-JF15
GenBank Accession LN506789 [GenBank]
Sequencing primer gene specific primer, JF15: 5'-CCGTCTACTTCAGAGGCTACAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K102180-0022-869-H08C-JF15
GCTACGAGCAGCATGAGATGGCTATTTGTTCTTTGACCTGTTAAAACTAGAGATGCTCTT
CTGGTAGGTCTTAATGCAACTTCTGTTTAGCTATGTAATGTTATGCTTTACCTAAAGACT
ACAAGTATGTTAGATGTTGTTACATCTATTCATATTATTTGAAGACTTTTGCATAAAAGT
TACTTTTGTTTCTTTCTGTTGAAACCATTAAAAGATTTGTTGGATCATGACAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCG

Last Updated on 06.12.2018 16:11