Sequences from confirmation amplicon


Confirmation sequences for line 234D05 and hit At3g16630 are shown.
Line ID 234D05
Locus At3g16630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5664071
Primers used for PCR 12ma - 8474, show primer details
   
Sequence Name 24-K114217-0022-234-D05C-12ma
GenBank Accession LN497086 [GenBank]
Sequencing primer gene specific primer, 12ma: 5'-TCAGTTATTGCCGAGTAGGAAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K114217-0022-234-D05C-12ma
CAGTTTGCTTGTTGGCCTGCAGAATATGAAGTTTCAGATGTACAAATTGTAAAGGATTTT
ATCGAGAAAGGAAATGCCGAAAGGAGCACAGGTTCAACTGGAGCAAATGAGGAATCTTCT
AGATCGCATGCCATCCTACAGCTTGTTGTAAAAAAGCATGTTGAGGTAAAAGACACTAGA
CGGAGGAATAATGATAGTAATGAATTGCCTGGGAAAGTTGTGGGAAAGATTTCTTTCATT
GACCTTGCTGGCAGTGAAAGAGGTGCAGACACCACAGACAATGATCGCCAGACAAGGTAA
AGATTCTCTTTGCCCCTGTGATTGAGTAGTTTGAGAATTGACCTACCACTAATGTGTAGA
TTAAAATTTATGCCTCTGTTGGACTTGTGGGCATTGATAAAGTGGCATTGAAAACAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCG
   
Sequence Name 06-K114216-0022-234-D05C-8409
GenBank Accession LN488904 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114216-0022-234-D05C-8409
TTGTTTTCATGCCACTTTATCAATGCCCACAAGTCCAACAGAGGCATAAATTTTAATCTA
CACATTAGTGGTAGGTCAATTCTCAAACTACTCAATCACAGGGGCAAAGAGAATCTTTAC
CTTGTCTGGCGATCATTGTCTGTGGTGTCTGCACCTCTTTCACTGCCAGCAAGGTCAATG
AAAGAAATCTTTCCCACAACTTTCCCAGGCAATTCATTACTATCATTATTCCTCCGTCTA
GTGTCTTTTACCTCAACATGCTTTTTTACAACAAGCTGTAGGATGGCATGCGATCTAGAA
GATTCCTCATTTGCTCCAGTTGAACCTGTGCTCCTTTCGGCATTTCCTTTCTCGATAAAA
TCCTTTACAATTTGTACATCTGAAACTTCATATTCTTGCAGGCCAACAATGCAAACTTGC
TGTCTACCATCTTCTCGCATGCAAAGTTTCCTACTCGCAAATAACTG

Last Updated on 06.12.2018 16:11