Sequences from confirmation amplicon


Confirmation sequences for line 541B11 and hit At3g22460 are shown.
Line ID 541B11
Locus At3g22460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7965093
Primers used for PCR 23qn - 8474, show primer details
   
Sequence Name 11-K115694-0022-541-B11C-23qn
GenBank Accession LN491261 [GenBank]
Sequencing primer gene specific primer, 23qn: 5'-CACTATAAGCTTCCCAGCATTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115694-0022-541-B11C-23qn
TGCTGCTCCGATGAGATTCCCACCTACAGTACATAGGAGGAGACAAATGTGATAACGATC
AAGGACCTGTTTTTATTTTCATGGTGAGGTTTGAATCATAATGGTGAGGATAAAGCTGAA
TCAAGACATTTTTGGCTTAACAGAAAAATCTGATGAGGCAAAATGTGAAGCACTTACTAA
CAGACCCTCTTCCCTAGCAAGAAGCCTTGCCATGTCAATGGATTCTTCACTTGAAACCTG
CGTAAGCGTTACAAAACTATAAGAGGCAACTAAGAAATGTAAGGTGTGTTTTGGAGTCAG
CAAGATGATATTGGTATGAAACCTTACCTGAACAACTTCATCTATAAGATCAACATCCAA
AATGCCTGGAATAAAACCAGCACCTATACCTTGAATTTTGTGGGGACCTGAGCATGATCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA
   
Sequence Name 02-K115693-0022-541-B11C-8409
GenBank Accession LN487170 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115693-0022-541-B11C-8409
TTGATCATGCTCAGGTCCCCACAAAATTCAAGGTATAGGTGCTGGTTTTATTCCAGGCAT
TTTGGATGTTGATCTTATAGATGAAGTTGTTCAGGTAAGGTTTCATACCAATATCATCTT
GCTGACTCCAAAACACACCTTACATTTCTTAGTTGCCTCTTATAGTTTTGTAACGCTTAC
GCAGGTTTCAAGTGAAGAATCCATTGACATGGCAAGGCTTCTTGCTAGGGAAGAGGGTCT
GTTAGTAAGTGCTTCACATTTTGCCTCATCAGATTTTTCTGTTAAGCCAAAAATGTCTTG
ATTCAGCTTTATCCTCACCATTATGATTCAAACCTCACCATGAAAATAAAAACAGGTCCT
TGATCGTTATCACATTTGTCTCCTCCTATGTACTGTAGGTGGGAATCTCATCTGGAGCAG
CAGCTACTGCAGCAATCAAACTTGCAAAGAGGCCAGAAAATGCTGGGAAGCTTATAGTG

Last Updated on 06.12.2018 16:11