Sequences from confirmation amplicon


Confirmation sequences for line 057H08 and hit At3g22960 are shown.
Line ID 057H08
Locus At3g22960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8141043
Primers used for PCR 19nj - 8474, show primer details
   
Sequence Name 53-K114716-0022-057-H08C-19nj
GenBank Accession LN508236 [GenBank]
Sequencing primer gene specific primer, 19nj: 5'-AGGACTGGTTGGATATTGATTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K114716-0022-057-H08C-19nj
CAGTCGGCTGAAGTCATTAATCACCTTAAAAGTTATCTTGCTGCTCGTTCCCGTGGAGGG
TAAGTAAACATGCTCAATATCAACACATCATACTACTGTACAGGAAACATATGATCTACT
TTGGGATTGGGTTTTGCGTCATGTATTCACCATCTTTAGTTATAGTTTTATTTTGATGTT
GTATTCAACTATGATGCAGGGAAATTGGAGTGATTGCAAAGATCGAGAGTATCGATTCAC
TGACCAATTTGGAAGAAATTATTCTAGCATCAGATGGGGCCATGGTTGCAAGAGGAGATC
TGGGAGCTCAGATACCTCTTGAGCAAGTTCCAGCAGCTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCGCAAGATTATA
   
Sequence Name 05-K114715-0022-057-H08C-8409
GenBank Accession LN488457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114715-0022-057-H08C-8409
ACTTGCTCAAGAGGTACTGAGCTCCCAGATCTCCTCTTGCACCATGGCCCCATCTGATGC
TAGAATAATTTCTTCCAAATTGGTCAGTGAATCGATACTCTCGATCTTTGCAATCACTCC
AATTTCCCTGCATCATAGTTGAATACAACATCAAAATAAAACTATAACTAAAGATGGTGA
ATACATGACGCAAAACCCAATCCCAAAGTAGATCATATGTTTCCTGTACAGTAGTATGAT
GTGTTGATATTGAGCATGTTTACTTACCCTCCACGGGAACGAGCAGCAAGATAACTTTTA
AGGTGATTAATGACTTCAGCCGACTTGACAAACGATACAGCAATGAAATCCACACCTTCA
GCAATTCCAAAATCAATATCCAACCAGTCC

Last Updated on 06.12.2018 16:11