Sequences from confirmation amplicon


Confirmation sequences for line 307D12 and hit At3g27280 are shown.
Line ID 307D12
Locus At3g27280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10077933
Primers used for PCR 09xu - 8474, show primer details
   
Sequence Name 42-K119622-0022-307-D12C-09xu
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 09xu: 5'-AATAAGGTAGACGGTGCTAGGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K119622-0022-307-D12C-09xu
GGTGGAGCAATTGTAATTTGAAGGGAAAAGTTAAAGTTTGTGAATGCACTATACTTAGAG
TTTGTCTACCCCACCACAAGAGGCCACCGCATGTACCGAAGCCACCAAAGTGACCACATA
AAAAAGTTTTATTTAATTTATTTACCATGTTTTAATTTATATTTTAAATAATACAAGTAC
AACAACCTTGGTGTTGGAAATTGTTTTTGACAATACAAATTTCGACTATTGTTATGGTAG
ATTTTTTGGTGAAGCCTTTCATTGGAATGATCGGGTTTCGACAACAATGGCATAGTCTTT
ACTTCTAGAGTCAGAAAAGATAGAACGGCTCAAAACAATTATCTTTTTTCACATCGTGTT
CATCAGAAATAAAAGAGCATCAACGTTAATAAACTTCACAGCTCCGTCGTTTTGTCTCCA
CTGAAGCTCCTCTTCCCACCTGCAAATACAACAAGGCCAAACAAGTAAACCACACCATTC
CACAAACCTAAACCATACTCTACTATCAAACCAAACAGTTTATACCAAACCATAACCACT
CTTAGTTCAACCAAACCGAATAACCTTAATGCCTTTAAATGGGAATCTTAAGTCCAAATC
CATTTTTCATTCATTATTCAAAGAAGTAAAAGAGCATACCAATGTTATAGCTCAACGACC
AGGGTTCAGATTAAAGAGCATGCTTTGTCCACCAGGTAAGTAAGCCACGTTCGGAGACCT
AGCCAGAGTAGCCGCAACCTCCCTTGAAGCTTCAATCCTCCTAAGCTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAATGTAGA
   
Sequence Name 18-K119620-0022-307-D12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119620-0022-307-D12C-8409
TTGAGCTAGGAGGATTGAAGCTTCAGGGAGGTTGCGGCTACTCTGGCTAGGTCTCCGAAC
GTGGCTTACTTACCTGGTGGACAAAGCATGCTCTTTAATCTGAACCCTGGTCGTTGAGCT
ATAACATTGGTATGCTCTTTTACTTCTTTGAATAATGAATGAAAAATGGATTTGGACTTA
AGATTCCCATTTAAAGGCATTAAGGTTATTCGGTTTGGTTGAACTAAGAGTGGTTATGGT
TTGGTATAAACTGTTTGGTTTGATAGTAGAGTATGGTTTAGGTTTGTGGAATGGTGTGGT
TTACTTGTTTGGCCTTGTTGTATTTGCAGGTGGGAAGAGGAGCTTCAGTGGAGACAAAAC
GACGGAGCTGTGAAGTTTATTAACGTTGATGCTCTTTTATTTCTGATGAACACGATGTGA
AAAAAGATAATTGTTTTGAGCCGTTCTATCTTTTCTGACTCTAGAAGTAAAGACTATGCC
ATTGTTGTCGAAACCCGATCATTCCAATGAAAGGCTTCACCAAAAAATCTACCATAACAA
TAGTCGAAATTTGTATTGTCAAAAACAATTTCCAACACCAAGGTTGTTGTACTTGTATTA
TTTAAAATATAAATTAAAACATGGTAAATAAATTAAATAAAACTTTTTTATGTGGTCACT
TTGGTGGCTTCGGTACATGCGGTGGCCTCTTGTGGTGGGGTAGACAAACTCTAAGTATAG
TGCATTCACAAACTTTAACTTTTCCCTTCAAATTACAATTTGCTCCACCATAACCTTCGT
TGTTACAACTCGTTCGGCACCTCCTAGCACCG

Last Updated on 06.12.2018 16:11