Sequences from confirmation amplicon


Confirmation sequences for line 039E12 and hit At3g48870 are shown.
Line ID 039E12
Locus At3g48870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18123827
Primers used for PCR PJ02 - 8474, show primer details
   
Sequence Name 11-K027486-022-039-E12C-8409
GenBank Accession LN490965 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K027486-022-039-E12C-8409
CCATTTACATTGAATATATCCTGAGAAACCGATAATGCTGCCATTTACATGTCGGGAAGA
AACCGATCACTGCTCATTCAAGAGAAGAAGCTGGGTTGAGATAATGCTGCAATTTGTGTA
AAGACAATGTTCTGTGTTGGTGAAAGACTGAAATTGAATAAAATAAAAACCTGATGTACT
GATGTGACAATTGTGCAGCAGCAACCAAGGCTTCATCAGTGTATCGAAGTTTGTGGTGGA
TCTCATAGCGCTCACGCAGACCTTGCAAAATCTGTATAGCTTCTTCTACAGTTGGTTCAG
GTACTTTCACAGGCTGGAACCGTCTCTCCAATGCAGGATCTTTCTCAATGTGTTTCCTGT
ACTCATCAATTGTTGTTGCACCAATACACTACAACCGTAAAAAAAGAAAAAAAAAACATT
TCGTAAATCTTGAGTTTACGGTTCTAATTGACATTTCGACAGCATTAAACTGATAAAAAG
AATTGAGAAAATTTACCTGCAATTCACCTCTTGCTAGAGCTGGCTTTAAAATGTTAGCAG
CATCGATCGCACCTTCAGCGGCTCCTGCACCGATAAGCGTGTGCACTTCATCAATAAACA
GAATTATCTCATCACTTTGCCTGATTTCCTCCATAAGCTTCTTCAATCTTTCCTCGAACT
CTCCACG
   
Sequence Name 12-K027486-022-039-E12C-PJ02
GenBank Accession LN491423 [GenBank]
Sequencing primer gene specific primer, PJ02: 5'-CTTCTAGTGGCTGGAACGAAATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K027486-022-039-E12C-pj2
TATGGAGGAAATCAGGCAAAGTGATGAGATAATTCTGTTTATTGATGAAGTGCACACGCT
CATCGGTGCAGGAGCCGCTGAAGGTGCGATCGATGCTGCTAACATCTTAAAGCCAGCTCT
AGCAAGAGGTGAATTGCAGGTAAATTTTCTCAATTCTTTTTATCAGTCTAATGCTGTCGA
AATGTCAATTAGAACCGTAAACTCAAGATTTACGAAATGTTTTTTTTTTCTTTTTTTACG
GTTGTAGTGTATTGGTGCAACAACAATTGATGAGTACAGGAAACACATTGAGAAAGATCC
TGCATTGGAAAGACGGTTCCAGCCTGTGAAAGTACCTGAACCAACTGTAAAAAAAGCTAT
ACAGATTTTGCAAGGTCTGCGTGAGCGCTATGAAATCCACCACAAACTTCGATACACTGA
TAAAGCCTTGGTTGCTGCTGCACAATTGTCACATCAGTACATCAGGTTTTTATTTTATTC
AATTTCAGTCTTTCACCAACACAAAACATTGTCTTTACACAAATTGCAGCATTATCTCAA
CCCAGCTTCTTCTCTTGAATGAGCAGTGATCGGTTTCTTCCCGACATGTAAATGGCAGCA
TTATCGGTTTCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATG
Primers used for PCR FL84 - 8474, show primer details
   
Sequence Name 77-K029747-022-039-E12C-8409
GenBank Accession LN513476 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K029747-022-039-E12C-8409
GCCATTTACAATTGAATATATCCATCATACTCATGCTCAGGTTTAATATTTGCATGTATC
TCAACTACCAAAGAACTATGTGTGTTTATATCTACTTGAGCTGATTCAGAGAAAGTTTCG
TTTGCTTAGCTTCCTGAGGAAGCTAGAGAGCTTGAAAAGCAACTCAGGCAAATCACCAAA
GAGAAGAATGAAGCTGTGCGAAGCCAAGACTTCGAGATGGTATCGATTTCAAGTATTTCT
TACTCTCTATACATCCAAGACTAATATTTACATTTCCTGAGTTCTTCTTTCGACAGGCTG
GTTCTCATCGTGACCGTGAAATAGAGCTCAAGGCTGAGATAGCTAATGTTTTATCTCGAG
GGCAAAG
   
Sequence Name 78-K029747-022-039-E12C-FL84
GenBank Accession LN513621 [GenBank]
Sequencing primer gene specific primer, FL84: 5'-TTCTTTGCCTCGAGATAAAACATT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K029747-022-039-E12C-FL84
GTCACGATGAGAACCAGCCTGTCGAAAGAAGAACTCAGGAAATGTAAATATTAGTCTTGG
ATGTATAGAGAGTAAGAAATACTTGAAATCGATACCATCTCGAAGTCTTGGCTTCGCACA
GCTTCATTCTTCTCTTTGGTGATTTGCCTGAGTTGCTTTTCAAGCTCTCTAGCTTCCTCA
GGAAGCTAAGCAAACGAAACTTTCTCTGAATCAGCTCAAGTAGATATAAACACACATAGT
TCTTTGGTAGTTGAGATACATGCAAATATTAAACCTGAGCATGAGTATGATGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTC

Last Updated on 06.12.2018 16:11