Sequences from confirmation amplicon


Confirmation sequences for line SALK_111366 and hit At3g56140 are shown.
Line ID SALK_111366
Locus At3g56140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20830295
Primers used for PCR AD05 - R204, show primer details
   
Sequence Name 46-K105819-SALK_111366C-AD05
GenBank Accession LN485406 [GenBank]
Sequencing primer gene specific primer, AD05: 5'-CCTGTCACCACTAACAGTAGACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K105819-SALK_111366C-AD05
TAACTTGTGACATTGTATGGTCTTCAACAACTCTTGCTTGTGCCGACAAATATGAACTCG
GTCCAAAAGGAACAATCTGTGTTGGGAGACTCATATCGAATGTAGATCTACGTGAGAATG
AAGAAAATCCTGAGATAAACCCAGAACCAGCAGGGGGAGTGTATAATTTACGCTCAGATT
TTGAAAGACCACGGATACCTTCTGCTTGGACAGTTCTTAAAACCTACATCAGATTAAGCA
GAGTGTTGTCAGCTCTTACCAGAGTCACAAAACAAGAGCAATATTTCTGATAAGCTCGCT
CTCTTTAGTCTTAACAGCTTAAACATGTCCATACTCATTTCAAATGATCAAAACCATATT
CATACCACTTACAAGTATCAAGATGTAATTAGTCTCACAAACTTCCATCAGATAGAAACC
AATGTAAAATGAAGCAGCATAGTTTAAAGGATTAGAAATTAAACACTGAACTAGGAAATA
ACACATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTG
GTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGA
GAGAGTTGCAGCA
   
Sequence Name 62-K105820-SALK_111366C-R210
GenBank Accession LN485781 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K105820-SALK_111366C-R210
GTGTTTATTTCTAATCCTTTAAACTATGCTGCTTCATTTTACATTGGTTTCTATCTGATG
GAAGTTTGTGAGACTAATTACATCTTGATACTTGTAAGTGGTATGAATATGGTTTTGATC
ATTTGAAATGAGTATGGACATGTTTAAGCTGTTAAGACTAAAGAGAGCGAGCTTATCAGA
AATATTGCTCTTGTTTTGTGACTCTGGTAAGAGCTGACAACACTCTGCTTAATCTGATGT
AGGTTTTAAGAACTGTCCAAGCAGAAGGTATCCGTGGTCTTTCAAAATCTGAGCGTAAAT
TATACACTCCCCCTGCTGGTTCTGGGTTTATCTCAGGATTTTCTTCATTCTCACGTAGAT
CTACATTCGATATGAGTCTCCCAACACAGATTGTTCCTTTTGGACCGAGTTCATATTTGT
CGGCACAAGCAAGAGTTGTTGAAGACCATACAATGTCACAAGTTATTTTACAAGCAGTAG
CAGATGGAGGGGGTACTGGTCTACTGTTATGNNTGACAGG
Primers used for PCR 29ah - LBb3, show primer details
   
Sequence Name 56-K119292-SALK_111366C-29ah
GenBank Accession LN485652 [GenBank]
Sequencing primer gene specific primer, 29ah: 5'-AACTCGAAATCGTTAGAAACCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K119292-SALK_111366C-29ah
ATCTCTGTACACTGGAAGCATTCCCTCTCGATTTGCAGGATCAGCTTAATCAATACATGG
ATAAGAGGTTTGTCTACACATAAACTTACTGCAAAATTCGAGATTGCCGCCGCGTACTTT
TCACGTGATCGTGAATATTGTTTGGTGGCAGGATGGACGGAGAGACATTGAAGTCTTATG
TGACACATTGGCCTGCTCAACGTTGGCAAGAATATGAGCCTCTTTTAAGTTATTGCCGCG
ATAACTCTGTTAGACTAATTGCTTGTGGCACTCCTCTCAAGGTAGAGTCTTGAAAGTATT
TGGACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTTTTTAATGTACTGGG
GTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCC
TGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTG
ATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 08-K119291-SALK_111366C-R210
GenBank Accession LN484509 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119291-SALK_111366C-R210
CATTTGTCCAATACTTTCAAGACTCTACCTTGAGAGGAGTGCCACAAGCAATTAGTCTAA
CAGAGTTATCGCGGCAATAACTTAAAAGAGGCTCATATTCTTGCCAACGTTGAGCAGGCC
AATGTGTCACATAAGACTTCAATGTCTCTCCGTCCATCCTGCCACCAAACAATATTCACG
ATCACGTGAAAAGTACGCGGCGGCAATCTCGAATTTTGCAGTAAGTTTATGTGTAGACAA
ACCTCTTATCCATGTATTGATTAAGCTGATCCTGCAAATCGAGAGGGAATGCTTCCAGTG
CTACAGAGATTTGACGCTCGCTCTCAACGCATCTCTTCCTCAGGTTTCTAACGATTTCGA
GT

Last Updated on 06.12.2018 16:11