Sequences from confirmation amplicon


Confirmation sequences for line 890E01 and hit At3g58120 are shown.
Line ID 890E01
Locus At3g58120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21521819
Primers used for PCR HP39 - 8474, show primer details
   
Sequence Name 66-K115259-0022-890-E01C-HP39
GenBank Accession LN511675 [GenBank]
Sequencing primer gene specific primer, HP39: 5'-GGTAATCGTATTCACGACCCTAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K115259-0022-890-E01C-HP39
ATTTTATTTACATTTCCCAAACCCCAAGGAAGGTCATGCATGCACGTACAGTTTATAATA
GTATCTGATAATTCGATACACCGNTTTTTATAAAATAGTTAAATTAGTGTCACATCGATA
AAATCTAATTTTTGTTTTTGCTGAAATTTTCTACAGAATTTTAGCAAATAGGCAATCAGC
ACAGAGATCAAGGGTGAGGAAATTGCAATACATATCAGAGCTTGAAAGGAGCGTTACTTC
ATTGCAGGTAATTAATTTACACACAAAGATATACTATGTTTATTAATGTAAAACAATCTA
AATAAAAAGTTTAAATACTGATCAACAAATCATTTAAAATTGTGGAATGAAGACTGAAGT
GTCAGTGTTATCGCCAAGAGTTGCGTTTTTGGATCATCAGCGATTGCTTCTCAACGTCGA
CAATAGTGCTATCAAGCAACGAATCGCAGCTTTAGCACAAGATAAGATTTTCAAAGACGG
TAATTTTCTATTTTGAACTTTTTTTCAATTTTCTAACATTGTATAAAATTCTTCTGAATA
TATTTTTCAGGGATACTATCAAACTATAGAAATTTGTTTGTTTTTTTTTACTATTATTGT
CTGAAGTTTCACTAGCTCCATGTATTCCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 26-K115258-0022-890-E01C-8409
GenBank Accession LN498057 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K115258-0022-890-E01C-8409
ATCCTGGAATACATGGAGCTAGTGAAACTTCAGACAATAATAGTAAAAAAAAACAAACAA
ATTTCTATAGTTTGATAGTATCCCTGAAAAATATATTCAGAAGAATTTTATACAATGTTA
GAAAATTGAAAAAAAGTTCAAAATAGAAAATTACCGTCTTTGAAAATCTTATCTTGTGCT
AAAGCTGCGATTCGTTGCTTGATAGCACTATTGTCGACGTTGAGAAGCAATCGCTGATGA
TCCAAAAACGCAACTCTTGGCGATAACACTGACACTTCAGTCTTCATTCCACAATTTTAA
ATGATTTGTTGATCAGTATTTAAACTTTTTATTTAGATTGTTTTACATTAATAAACATAG
TATATCTTTGTGTGTAAATTAATTACCTGCAATGAAGTAACGCTCCTTTCAAGCTCTGAT
ATGTATTGCAATTTCCTCACCCTTGATCTCTGTGCTGATTGCCTATTTGCTAAAATTCTG
TAGAAAATTTCAGCAAAAACAAAAATTAGATTTTATCGATGTGACACTAATTTAACTATT
TTATAAAAACGGTGTATCGAATTATCAGATACTATTATAAACTGTACGTGCATGCATGAC
CTTCCTTGGGGTTTTGGGAAATAGTAAATAAAAATGTTAATTACCTTTTTACCCTTTTAG
GGTCGTGA

Last Updated on 06.12.2018 16:11