Sequences from confirmation amplicon


Confirmation sequences for line SALK_047914c and hit At4g14620 are shown.
Line ID SALK_047914c
Locus At4g14620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8389669
Primers used for PCR 40vi - LBb3, show primer details
   
Sequence Name 38-K117945-SALK_047914C-40vi
GenBank Accession LN485272 [GenBank]
Sequencing primer gene specific primer, 40vi: 5'-TTTAACGGGAACAATGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K117945-SALK_047914C-40vi
CATTTCAGGTATATATAAAAAACTCAATCTGACTCTGTTTCAATCTCTGCAACAATCTAA
AATCTTCATGGATTTGATTACAGAGTTTGATTCAATGCGGAAGCTTCGTTGAGAAGAGTC
TTTTAGTGGAAGCTACGAAGATTATAGAGAAGAACAAATCAGTTAAAAGAAAAGACGAAT
TGAGAAAGATTGTTGTTGATGAACTCTCATCTCTTGGTTACGACTCTTCAATTTGTAAAT
CGAAATGGGATAAAACTCGTTCTATACCGGCCGGTATAATCTCCTTAACCAGTTTTTCTT
CTTCTTCTTCTACCTTTGATTTTTGTGATTTTTCGTGTAATTGAAGTGTTTTTATTGTTG
ATGATTTTTCAGGTGAGTATGAGTATATAGATGTGATTGTGAACGGAGAGAGGTTGATAA
TCGACATTGATTTTAGATCGGAGTTTGAGATCGCAAGGCAGACGTTTAGACAACTTAATA
ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC
AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG
GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAA
   
Sequence Name 03-K117944-SALK_047914C-R210
GenBank Accession LN484352 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117944-SALK_047914C-R210
CTGCGATCTCAAACTCCGATCTAAATCAATGTCGATTATCAACCTCTCTCCGTTCACAAT
CACATCTATATACTCATACTCACCTGAAAAATCATCAACAATAAAAACACTTCAATTACA
CGAAAAATCACAAAAATCAAAGGTAGAAGAAGAAGAAGAAAAACTGGTTAAGGAGATTAT
ACCGGCCGGTATAGAACGAGTTTTATCCCATTTCGATTTACAAATTGAAGAGTCGTAACC
AAGAGATGAGAGTTCATCAACAACAATCTTTCTCAATTCGTCTTTTCTTTTAACTGATTT
GTTCTTCTCTATAATCTTCGTAGCTTCCACTAAAAGACTCTTCTCAACGAAGCTTCCGCA
TTGAATCAAACTCTGTAATCAAATCCATGAAGATTTTAGATTGTTGCAGAGATTTGAAAC
AGAGTCAGATTGAGTTTTTTTTATATATACCTTGAAATTGTCATAATCGAAGAAATCGAG
TTCATCGTCGGAGATATCATTGTTCCCCGTTAAA

Last Updated on 06.12.2018 16:11