Sequences from confirmation amplicon


Confirmation sequences for line 051G05 and hit At4g17430 are shown.
Line ID 051G05
Locus At4g17430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9726690
Primers used for PCR CW49 - 8474, show primer details
   
Sequence Name 74-K104358-0022-051-G05C-CW49
GenBank Accession LN513069 [GenBank]
Sequencing primer gene specific primer, CW49: 5'-TTGTTTAAAATGTTATGTGGACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K104358-0022-051-G05C-CW49
TCAAAATTAACTATGTTTAAGTTCGTATTATTTTAGTTAAGGCCCATCAGAGAATATTAG
TTAGGCCCAAAACAGTGACACTCACCGTTTTGGCATCTTCTACACATGAGCAAAACTAGA
CGCAACGCAATGATGGCGACTAAAACCTCAGAGCAAAAAACATGAACAACTTCTTCAATC
CCAGTAGACCAAGCCCACGTCCATGGCCAAACAGGAAGAAGCAAACCGACAAATCTGCGA
TATTTCTTTGCTCCGTTTCTATACTTGTTGTGTAAATATATTCAAGATGGTCAAGTCCGA
TGAGTAAATGGCTTACTCAATCACTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 82-K104359-0022-051-G05C-8409
GenBank Accession LN514188 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K104359-0022-051-G05C-8409
GTGATTGAGTAAGCCATTTACTCATCGGACTTGACCATCTTGAATATATTTACACAACAA
GTATAGAAACGGAGCAAAGAAATATCGCAGATTTGTCGGTTTGCTTCTTCCTGTTTGGCC
ATGGACGTGGGCTTGGTCTACTGGGATTGAAGAAGTTGTTCATGTTTTTTGCTCTGAGGT
TTTAGTCGCCATCATTGCGTTGCGTCTAGTTTTGCTCATGTGTAGAAGATGCCAAAACGG
TGAGTGTCACTGTTTTGGGCCTAACTAATATTCTCTGATGGGCCTTAACTAAAATAATAC
GAACTTAAACATAGTTAATTTTAGAAATCTTTTTGTAACATAATCTATACGAAGCTATTT
GCGCGTCCACATAACATTTTAAACA

Last Updated on 06.12.2018 16:11