Sequences from confirmation amplicon


Confirmation sequences for line 255G08 and hit At4g22720 are shown.
Line ID 255G08
Locus At4g22720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11936852
Primers used for PCR MS60 - 8474, show primer details
   
Sequence Name 34-K017191-022-255-G08C-MS60
GenBank Accession LN501214 [GenBank]
Sequencing primer gene specific primer, MS60: 5'-CCTACAAGCGGTTATGAAAGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K017191-022-255-G08C-MS60
GGCTTCCCCTACGCTTCCTCTTCTTGTTCCTCACAGAAACAGCGAAACCTCGGTGGTTGC
GGGATACACTGTGCCTAAAGACTCCAAGATTTTCATCAACGTTTGGGCGATTCATAGAGA
TCCTAAGAATTGGGATGAACCAAACGAGTTCAAACCTGAGAGGTTTTTGGAGAATTCGCT
TGATTTCAATGGAGGTGATTTTAAGTATTTGCCTTTTGGGTCAGGAAGGAGGATTTGTGC
GGCGATAAACATGGCTGAGAGACTTGTTCTGTTCAACATTGCTTCACTTCTTCACTCTTT
TGATTGGAAAGCTCCTCAAGGACAGAAGTTTGAGGTTGAAGAGAAGTTTGGTCTTGTTCT
TAAGTTGAAGTCTCCACTTGTGGCTATTCCTGTTCCAAGGTTGTCCGATCCAAAACTCTA
CACAGCTTAAGTAAGAAGGATGTTGAATTGAGTTTTCTTGTTTAAAGTTTACTTTTCTTT
ATCTGTGTCTGGAGTTTTGATGCTTTGTATAAAAGATGTGTCAAGTTACATTTTTGATAA
CATTTAATATAACAAAGTTTTACTTACAATAATAACGCCTAATAGTCGATGCCAAAGCTA
TAAAACTATATTGTAGTTATATTTTTGGCAAGTCGATTAAAATTCTTGGGTCAACAAATT
ACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCCATTTTTTTTCAATT
   
Sequence Name 38-K017191-022-255-G08C-8409
GenBank Accession LN502810 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K017191-022-255-G08C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGTAATTTGTTGACCCAAGAATTTTAATC
GACTTGCCAAAAATATAACTACAATATAGTTTTATAGCTTTGGCATCGACTATTAGGCGT
TATTATTGTAAGTAAAACTTTGTTATATTAAATGTTATCAAAAATGTAACTTGACACATC
TTTTATACAAAGCATCAAAACTCCAGACACAGATAAAGAAAAGTAAACTTTAAACAAGAA
AACTCAATTCAACATCCTTCTTACTTAAGCTGTGTAGAGTTTTGGATCGGACAACCTTGG
AACAGGAATAGCCACAAGTGGAGACTTCAACTTAAGAACAAGACCAAACTTCTCTTCAAC
CTCAAACTTCTGTCCTTGAGGAGCTTTCCAATCAAAAGAGTGAAGAAGTGAAGCAATGTT
GAACAGAACAAGTCTCTCAGCCATGTTTATCGCCGCACAAATCCTCCTTCCTGACCCAAA
AGGCAAATACTTAAAATCACCTCCATTGAAATCAAGCGAATTCTCCAAAAACCTCTCAGG
TTTGAACTCGTTTGGTTCATCCCAATTCTTAGGATCTCTATGAATCGCCCAAACGTTGAT
GAAAATCTTGGAGTCTTTAGGCACAGTGTATCCCGCAACCACCGAGGTTTCGCTGTTTCT
GTGAGGAACAAGAAGAGGAAGCGTAGGGTGAAGCCTAAGAGTCTCTTTCA

Last Updated on 06.12.2018 16:11