Sequences from confirmation amplicon


Confirmation sequences for line 154C07 and hit At4g23920 are shown.
Line ID 154C07
Locus At4g23920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12431809
Primers used for PCR MP02 - 8474, show primer details
   
Sequence Name 23-K017119-022-154-C07C-MP02
GenBank Accession LN496348 [GenBank]
Sequencing primer gene specific primer, MP02: 5'-GTTTAGGCTTACAAATCCATCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K017119-022-154-C07C-MP02
TCCATTCGCTTTGTTAAATCTTAATTGAGCCTAATAAAATAACATTTGTTACCAAAAAAA
GAAGATCAACTATATAAATACACTTCCATTAACAAAAACGTCAAAGGGTAATTAAGACAA
TTCACAACATTCCGGTATATTCGCAACCAAACCAAATCCTGCCACGTAGCAATTATTTCC
TCTTCATTTTTCTCTCTGTCTCCCTCGAAGAAACGTAGAAATCAGAAAGTAAATCATACA
GTCAAAACCGCTTCTTCTATAAAACCCTTTTTAGCTCAATCCCAAAACGCAATAAAAAAC
AAAACGCTCTCCAGAATTTCGAAACTTCTAAGATATTTTGTTTTCTCTCTTAACGATGGC
GAAGAGTGTTTTGGTTACCGGCGGCGCTGGCTATATCGGAAGTCATACTGTTCTTCAACT
GCTTGAAGGTGGTTACTCCGCCGTCGTTGTTGACAATTACGACAATTCCTCTGCTGCTTC
GCTTCAGCGCGTGAAGAAACTCGCCGGCGAAAACGGAAACCGCCTCTCCTTCCACCAGGT
TCTGTTTTCGTTCTCTCTCTCCGCGGAAATGAAAAATTCTCGGTGCTGATTTGGTTTAGG
AAACGTGTGCTTGAAGAATTTCGTATCATCGTTTTCATATGGTTCTGGTTAATATTTGTG
TACACTATGTGGCTAAGTTTTCTTTGTGTGTACACATAATCATGAATTAAGTATTGAT
   
Sequence Name 24-K017119-022-154-C07C-8409
GenBank Accession LN496790 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K017119-022-154-C07C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATAAATATTAAATTACTCTTTCACAT
GGCATCATCAATACTTAATTCATGATTATGTGTACACACAAAGAAAACTTAGCCACATAG
TGTACACAAATATTAACCAGAACCATATGAAAACGATGATACGAAATTCTTCAAGCACAC
GTTTCCTAAACCAAATCAGCACCGAGAATTTTTCATTTCCGCGGAGAGAGAGAACGAAAA
CAGAACCTGGTGGAAGGAGAGGCGGTTTCCGTTTTCGCCGGCGAGTTTCTTCACGCGCTG
AAGCGAAGCAGCAGAGGAATTGTCGTAATTGTCAACAACGACGGCGGAGTAACCACCTTC
AAGCAGTTGAAGAACAGTATGACTTCCGATATAGCCAGCGCCGCCGGTAACCAAAACACT
CTTCGCCATCGTTAAGAGAGAAAACAAAATATCTTAGAAGTTTCGAAATTCTGGAGAGCG
TTTTGTTTTTTATTGCGTTTTGGGATTGAGCTAAAAAGGGTTTTATAGAAGAAGCGGTTT
TGACTGTATGATTTACTTTCTGATTTCTACGTTTCTTCGAGGGAGACAGAGAGAAAAATG
AAGAGGAAATAATTGCTACGTGGCAGGATTTGGTTTGGTTGCGAATATACCGGAATGTTG
TGAATTGTCTTAATTACCCTTTGACGTTTTTGTTAATGGAAGTGTATTTATATAGTTGAT
CTTCTTTTTTTGGTAACAAATGTTATTTTATTAGGCTC

Last Updated on 06.12.2018 16:11