Sequences from confirmation amplicon


Confirmation sequences for line SALK_099763c and hit At4g32130 are shown.
Line ID SALK_099763c
Locus At4g32130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15520601
Primers used for PCR 25kh - LBb3, show primer details
   
Sequence Name 72-K113949-SALK_099763C-25kh
GenBank Accession LN486060 [GenBank]
Sequencing primer gene specific primer, 25kh: 5'-ATAAACCAATGTTAGATCGCTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K113949-SALK_099763C-25kh
CCTGGATATTTTTTACTGGTACAAGTGAAAACCCCTGAGTCTTGGACTGTTGGTTAACGC
TTAGCGGCTAGCGGGTAACAAGCTAGTGAGTGAAGGCACTCCTTGGCTTCTCATCTGCTC
TTGTGCGCTCTTCATTTCCTCGGGATCTGTTTATCACACATACCAACAATTCTCATCAAA
CCATTTACATCTAATTTGCTAGGTTTCAATTCAGCAATAGCTTGAAGAGATTTATGAAAT
GATAAAAAAACAAGGCACTGCTAGAATCCTCAGTAGATTAGGTACACTGTTAGGTTGGTT
ATTTGTCGCATTGATTTGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACT
TAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGA
CGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCA
CGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATNGGGCAAAA
   
Sequence Name 61-K113948-SALK_099763C-R210
GenBank Accession LN485769 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113948-SALK_099763C-R210
TTTGTTTACCCACAATATATCCTGCAATCAATGCGACAAATAACCAACCTAACAGTGTAC
CTAATCTACTGAGGATTCTAGCAGTGCCTTGTTTTTTTATCATTTCATAAATCTCTTCAA
GCTATTGCTGAATTGAAACCTAGCAAATTAGATGTAAATGGTTTGATGAGAATTGTTGGT
ATGTGTGATAAACAGATCCCGAGGAAATGAAGAGCGCACAAGAGCAGATGAGAAGCCAAG
GAGTGCCTTCACTCACTAGCTTGTTACCCGCTAGCCGCTAAGCGTTAACCAACAGTCCAA
GACTCAGGGGTTTTCACTTGTACCAGTTAAAAAATATTCCAGGATAGACTTGGAAGAAAT
CAGCGTCAAGCAAATTCCCCAAGCGATCTAACNTTGGTTTA

Last Updated on 06.12.2018 16:11