Sequences from confirmation amplicon


Confirmation sequences for line 049B12 and hit At4g33000 are shown.
Line ID 049B12
Locus At4g33000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15924695
Primers used for PCR JK14 - 8474, show primer details
   
Sequence Name 09-K020436-022-049-B12C-8409
GenBank Accession LN489980 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K020436-022-049-B12C-8409
AGATTTCCCGGACATGTAGAGAATGGTTCAAATGACGGATATATTAGCGTGACATTTTGA
TAAACTGAAAAAATAATAAGTGAAGCAGAATCAAAGTTGCCACTTAGTCCGGAAGACTGT
GATTGTGATTTTGAATGAACTCATGGAACAAGTTTCCTCTGTAAATTTTTCTCACTACTT
TGATTTCTCATTTCTTCTCTGATTTTGTCTTTTTGTAATGTTCTTGTTCTTGTTTTGTTT
TGGTGAAACAGAGATCAAGCTCTCTCACTGTCGGAGAGCAATTCTGCGCCGTCTTTATAC
CTTTCTTTGCCATTATCGATGTCCTAGTTTCCTCCGTCGGACAATGCTTCGATTGCCGCA
GCACATCGCCTCGGACATGTCAGCACGCGGATCTGGAACGTCTTGCTCGTGAATCTCAAT
GTTGGTTTTCTTTTTGTTTTTGCCTCTCTCTCTCT
   
Sequence Name 10-K020436-022-049-B12C-JK14
GenBank Accession LN490452 [GenBank]
Sequencing primer gene specific primer, JK14: 5'-GAGAGAGAGAGAGGCAAAAACAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K020436-022-049-B12C-JK14
CAACATTGAGATTCACGAGCAAGACGTTCCAGATCCGCGTGCTGACATGTCCGAGGCGAT
GTGCTGCGGCAATCGAAGCATTGTCCGACGGAGGAAACTAGGACATCGATAATGGCAAAG
AAAGGTATAAAGACGGCGCAGAATTGCTCTCCGACAGTGAGAGAGCTTGATCTCTGTTTC
ACCAAAACAAAACAAGAACAAGAACATTACAAAAAGACAAAATCAGAGAAGAAATGAGAA
ATCAAAGTAGTGAGAAAAATTTACAGAGGAAACTTGTTCCATGAGTTCATTCAAAATCAC
AATCACAGTCTTCCGGACTAAGTGGCAACTTTGATTCTGCTTCACTTATTATTTTTTCAG
TTTATCAAAATGTCACGCTAATATATCCGTCATTTGAACCATTCTCTACATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11