Sequences from confirmation amplicon


Confirmation sequences for line 167D03 and hit At4g40050 are shown.
Line ID 167D03
Locus At4g40050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18563303
Primers used for PCR 13vf - 8474, show primer details
   
Sequence Name 45-K118077-0022-167-D03C-13vf
GenBank Accession LN505677 [GenBank]
Sequencing primer gene specific primer, 13vf: 5'-TTCCAGAAAAACAAACAAACAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K118077-0022-167-D03C-13vf
AGAGAGCATCACGAAACTTAAACAATTTCTTAGCGTGGAAGCGCGCAAGATAAGTTTGAG
AAGCTGGATATGAATCAAGCATTGCACAGTAACGTAGAGGCCTGAGATAAGTTAAATTTG
TATCAGATTCTATAAAGCGAGTGATTTCTTGCACAACTAGCCTCCACTCCTTAAAGTTTG
TCTCCTACACAAGAGAAGAAATATGTATAAAAAAAGAGACTTGAACCAATATAAATCACA
ACAAGGGTCAGATCAAAAGTACCCTAAAATTGGATATGCTGTGATCAACCAAAAGCCTAA
GCTTGTCAGCTAAGTGCAAAAGCAGCGTTACCAATTTACATGGGAAACATATAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 20-K118076-0022-167-D03C-8409
GenBank Accession LN495405 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118076-0022-167-D03C-8409
TGTTTCCATGTAAATTGGTAACGCTGCTTTTGCACTTAGCTGACAAGCTTAGGCTTTTGG
TTGATCACAGCATATCCAATTTTAGGGTACTTTTGATCTGACCCTTGTTGTGATTTATAT
TGGTTCAAGTCTCTTTTTTTATACATATTTCTTCTCTTGTGTAGGAGACAAACTTTAAGG
AGTGGAGGCTAGTTGTGCAAGAAATCACTCGCTTTATAGAATCTGATACAAATTTAACTT
ATCTCAGGCCTCTACGTTACTGTGCAATGCTTGATTCATATCCAGCTTCTCAAACTTATC
TTGCGCGCTTCCACGCTAAGAAATTGTTTAAGTTTCGTGATGCTCTCTTGGCAAGTTATC
ACCGTAATGAGGTAAACAAGCACTGTTTGTTTGTTTTTTCTGGA

Last Updated on 06.12.2018 16:11