Sequences from confirmation amplicon


Confirmation sequences for line 410E04 and hit At5g19025 are shown.
Line ID 410E04
Locus At5g19025 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6355424
Primers used for PCR RK96 - 8474, show primer details
   
Sequence Name 33-K023620-022-410-E04C-8409
GenBank Accession LN500830 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K023620-022-410-E04C-8409
GATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGGTGCATTGCTTTATT
TGGTGATTCCTAAATTCATCATAGCTGCTTAGAATTCTTGATTTTAATATCTCTTTAGGA
TTCCATTTTGATTCACTGTGACTAGTATGATTGTCCAATCTTCTGTTGTGTATCTTACTT
CGTTTGCAAGTATTCTCTGCAGATCGCCGGCAAAATTAGCTGTAAAGGGATGCATAGACA
ATCGCTGATCCAGAAACTTTCCAATCTATACTTACATATTATCACATTGAAATGTTGCAG
TTGCAGAGGTTCTTCTTCATCTTCTCGGATAACATTCATTTAGTATTAGGAGGAAAAATA
TAAAATTATTGATTTTTTTCATTTCCTATAGAGTTAAATCCCTGAAACTCAAATGTCTTG
TTACTCTCGAACACTTTCTCTGTGTAACTATATATACACTCAAGAAATATCTAATTCGAA
TGATCAAATCTAATGCCTTGTGTTGTATATTTGTAGTCTTTGTTTCACATAAAATAATGT
AAAACCTATATATACACTGTGTGAGCTATAGAAGACGAGAGAGAAGTATAAAAAAAAGTC
AAGCTTTAAGGTTTAGCTTTGTTGC
   
Sequence Name 34-K023620-022-410-E04C-RK96
GenBank Accession LN501254 [GenBank]
Sequencing primer gene specific primer, RK96: 5'-GAGATCATGAGAGCAACAAAGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K023620-022-410-E04C-Rk96
GCTTGACTTTTTTTTATACTTCTCTCTCGTCTTCTATAGCTCACACAGTGTATATATAGG
TTTTACATTATTTTATGTGAAACAAAGACTACAAATATACAACACAAGGCATTAGATTTG
ATCATTCGAATTAGATATTTCTTGAGTGTATATATAGTTACACAGAGAAAGTGTTCGAGA
GTAACAAGACATTTGAGTTTCAGGGATTTAACTCTATAGGAAATGAAAAAAATCAATAAT
TTTATATTTTTCCTCCTAATACTAAATGAATGTTATCCGAGAAGATGAAGAAGAACCTCT
GCAACTGCAACATTTCAATGTGATAATATGTAAGTATAGATTGGAAAGTTTCTGGATCAG
CGATTGTCTATGCATCCCTTTACAGCTAATTTTGCCGGCGATCTGCAGAGAATACTTGCA
AACGAAGTAAGATACACAACAGAAGATTGGACAATCATACTAGTCACAGTGAATCAAAAT
GGAATCCTAAAGAGATATTAAAATCAAGAATTCTAAGCAGCTATGATGAATTTAGGAATC
ACCAAATAAAGCAATGCACCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGAATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC

Last Updated on 06.12.2018 16:11