Sequences from confirmation amplicon


Confirmation sequences for line 384E01 and hit At5g61770 are shown.
Line ID 384E01
Locus At5g61770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:24817215
Primers used for PCR AM18 - 8474, show primer details
   
Sequence Name 13-K105747-0022-384-E01C-AM18
GenBank Accession LN492039 [GenBank]
Sequencing primer gene specific primer, AM18: 5'-TTCAGCAATGTGATTTTGTTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K105747-0022-384-E01C-AM18
GCTCCCCCTGCAGAGATAGTACTCTTGAACTACAACAAAGACACCAAGCTCATTGATTTC
CGGCATTATTCTATAAGACTTCAGCCTGTTGGAGTATCTCGTAGGATCAGAAAGTTTGTG
CAAAATCATCAGGTTCCAGATCTTAGGAATCTGCAGGATGTTAGTGATTTTGTCACCAAG
TAAGGATCTACATTGGTTCAGTCTGTTTGCTGCATCGATGTGGATGGATTAATGTGACAT
TTCGTCTTTCTTTTTTGTTGTCCCTTTTTGTCAGGGCTGGTTACGGATCAGAGAGCGAAG
GAGATGAAGAAGCGGCAACAGTGACACTATCATCTGATCTAGGCCGAGTTAACAAAGGTG
CCACCAAGAGCGCAGTGAAACTGCAAGAAATTGGACCAAGAATGGCTTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 29-K105748-0022-384-E01C-8409
GenBank Accession LN499290 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K105748-0022-384-E01C-8409
ATTGAAGCCATTCTTGGTCCATTTCTTGCAGTTTCACTGCGCTCTTGGTGGCACCTTTGT
TAACTCGGCCTAGATCAGATGATAGTGTCACTGTTGCCGCTTCTTCATCTCCTTCGCTCT
CTGATCCGTAACCAGCCCTGACAAAAAGGGACAACAAAAAAGAAAGACGAAATGTCACAT
TAATCCATCCACATCGATGCAGCAAACAGACTGAACCAATGTAGATCCTTACTTGGTGAC
AAAATCACTAACATCCTGCAGATTCCTAAGATCTGGAACCTGATGATTTTGCACAAACTT
TCTGATCCTACGAGATACTCCAACAGGCTGAAGTCTTATAGAATAATGCCGGAAATCAAT
GAGCTTGGTGTCTTTGTTGTAGTTCAAGAGTACTAATCTCTGGCAGGTGGAGAGCTTGAC
CTGATATATTTCACCATCAGAAGAACAAAATCCATTTGCTGA

Last Updated on 06.12.2018 16:11