Sequences from confirmation amplicon


Confirmation sequences for line 046C05 and hit F4I1 are shown.
Line ID 046C05
Locus F4I1 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18359024
Primers used for PCR SZ12 - 8474, show primer details
   
Sequence Name 01-K024916-022-046-C05C-8409
GenBank Accession LN486421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K024916-022-046-C05C-8409
CATGTAGATTTCTCTTTTATCATGTTCGTGTTACTTTATTTATTTTTATTCTTACTCTGA
ACAGAAATACAAGGTAAAAGTCGTGAGGATTTACCTATATATAATGGGCTCCCCAATATT
AATAAATAAACCATTAGAGTGGTAATTGACCGACACTTGGTTTGCCCTGCTAGTTGCTAG
ATCATACGTTAAACCCTTTTACTTTGTTTGTTGTCTTACTTTTTTGTTCCTTCTCGTCAC
ACCACCTCAAAAATTTTAAAATAAATTAAGTTTTTGATATATATTAAGTACAAAGAAACA
TAACCAGCGAAGTCGATGAGGTTATGTTTCTAACCATCTCAAGAAACTAATAACCCTCAA
ACCAATTTTACTAGATCCATTTCTAACCGTCTCAAGAAACTAAAAGCCCTCAAATCAATT
TTCAATATAGGAAATTGTGTAA
   
Sequence Name 02-K024916-022-046-C05C-SZ12
GenBank Accession LN486877 [GenBank]
Sequencing primer gene specific primer, SZ12: 5'-CCATTTCGTTTTACACAATTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K024916-022-046-C05C-SZ12
TTGAAAATTGATTTGAGGGCTTTTAGTTTCTTGAGACGGTTAGAAATGGATCTAGTAAAA
TTGGTTTGAGGGTTATTAGTTTCTTGAGATGGTTAGAAACATAACCTCATCGACTTCGCT
GGTTATGTTTCTTTGTACTTAATATATATCAAAAACTTAATTTATTTTAAAATTTTTGAG
GTGGTGTGACGAGAAGGAACAAAAAAGTAAGACAACAAACAAAGTAAAAGGGTTTAACGT
ATGATCTAGCAACTAGCAGGGCAAACCAAGTGTCGGTCAATTACCACTCTAATGGTTTAT
TTATTAATATTGGGGAGCCCATTATATATAGGTAAATCCTCACGACTTTTACCTTGTATT
TCTGTTCAGAGTAAGAATAAAAATAAATAAAGTAACACGAACATGATAAAAGAGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11