Sequences from confirmation amplicon


Confirmation sequences for line 038F12 and hit MQJ2 are shown.
Line ID 038F12
Locus MQJ2 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23636696
Primers used for PCR 04xm - 8474, show primer details
   
Sequence Name 26-K119249-0022-038-F12C-04xm
GenBank Accession LN498152 [GenBank]
Sequencing primer gene specific primer, 04xm: 5'-TAGAAGCAATAACATAAGGGACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K119249-0022-038-F12C-04xm
TACCAAACTCCCTGCATATCTCTTTAATATTTTACACAAGATCTATATTTCTTGAAGATT
AGTTTTAAAAAGAATTCTTACATATTAAACTACATTTCATACATGTCTTTTTAGTCGTTT
TTATTTTCATTTTACACTCACCATCTTCCACTTTAAAACACTTCTCTAAAGTTTTAAACC
AATGATAATTTTATTTTATTTTTTATAGTGTGAGATAATGTATTCTATTAATTATACATT
TATAATTTGTAGTTCACAATTCATTTACCGTAATCAATGTAGAATACATAGAAATAACAA
TAAAATCAATATATATGAAACAAAAGCAAAAACAAGAAAACCGTTTGTGATGAAATGGAT
GTCATGAAATTTTGTTGTTGTTGTGGACATAAATATTGATTTTATTGATATGATAACATT
ACAAGATTCTGAAATAGTCACTACGTACTAATAATAAATTTGGAAATAAAGGAGAAGTGG
AGATCCCACCTACGCCAAATATTGAATGCATCACCGCTTTTTGCGTTCTTCCACATACTG
GATTATTTCATTTTATCCTTTTTATTTTCATACATTCTCACTTATCGATCTCATTAAATA
TTTCCTTCGTTTCCTTTTAATCTCAAGGACTTGTCTAACCGGTGACTAGCTTGTGGCCGT
GCTGAGTTTGCATCGTCGAACAAAACTCATAAAATCCTATCTGACGCAGTCGCACAACAG
TTTATAATTAGACGTAATTCGTTGACAAATTGCCCTATAGATATATACAGTATACATTAA
CTAAATCTATCCTCTATCACCTCCCTATTTCTTAGTAAACAATAAAAGAAAGAAATAAAG
TTATTGTAAATGGCTTCATGTCCGGGGAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAGAGTATTTACCAATTTTTTTCATTCAAAATG
   
Sequence Name 10-K119248-0022-038-F12C-8409
GenBank Accession LN490890 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K119248-0022-038-F12C-8409
ATAACTTTATTTCTTTCTTTATTGTTTACTAAGAAATAGGGAGGTGATAGAGGATAGATT
TAGTTAATGTATACTGTATATATCTATAGGGCAATTTGTCAACGAATTACGTCTAATTAT
AAACTGTTGTGCGACTGCGTCAGATAGGATTTTATGAGTTTTGTTCGACGATGCAAACTC
AGCACGGCCACAAGCTAGTCACCGGTTAGACAAGTCCTTGAGATTAAAAGGAAACGAAGG
AAATATTTAATGAGATCGATAAGTGAGAATGTATGAAAATAAAAAGGATAAAATGAAATA
ATCCAGTATGTGGAAGAACGCAAAAAGCGGTGATGCATTCAATATTTGGCGTAGGTGGGA
TCTCCACTTCTCCTTTATTTCCAAATTTATTATTAGTACGTAGTGACTATTTCAGAATCT
TGTAATGTTATCATATCAATAAAATCAATATTTATGTCCACAACAACAACAAAATTTCAT
GACATCCATTTCATCACAAACGGTTTTCTTGTTTTTGCTTTTGTTTCATATATATTGATT
TTATTGTTATTTCTATGTATTCTACATTGATTACGGTAAATGAATTGTGAACTACAAATT
ATAAATGTATAATTAATAGAATACATTATCTCACACTATAAAAAATAAAATAAAATTATC
ATTGGTTTAAAACTTTAGAGAAGTGTTTTAAAGTGGAAGATGGTGAGTGTAAAATGAAAA
TAAAAACGACTAANAAGACATGTATGAAATGTAGTTTAATATGTAAGAATTCTTTTTAAA
ACTAATCTTCAAGAAATATAGATCTTGTGTAAAATATATAAAGAGATATGCATGGGAGTT
TGTGTTAGCCAGCTACCACGGCCGTACCTTGCACGTCCCGGCGGTCCC

Last Updated on 06.12.2018 16:11